Skip to main content

Advertisement

The effector protein ExoY secreted by Pseudomonas aeruginosa is a nucleotidyl cyclase with preference for GTP

Background

Pseudomonas aeruginosa is an important opportunistic pathogen causing serious pulmonary, urogenital and systemic infections. P. aeruginosa injects four effector proteins into host cells via the type III secretion system. ExoY is one of these proteins.

ExoY was originally classified as adenylyl cyclase with homology to the typical calmodulin-stimulated adenylyl cyclase exotoxins CyaA from Bordetella pertussis and edema factor from Bacillus anthracis, but the pathophysiologial function of ExoY has remained elusive [1, 2]. Recently, our group showed that CyaA and edema factor possess a rather broad base specifity (ATP >> CTP > UTP) [3, 4], raising the question of wether ExoY may also bind and metabolize nucleoside 5’- triphosphates other than ATP.

Methods

We determined cyclic nucleotide concentrations in cells transfected with ExoY plasmid or infected with P. aeruginosa with a highly sensitive HPLC-MS/MS method. Moreover, we determined the catalytic activity of purified ExoY.

Results

In mammalian cells transfected with ExoY plasmid and infected with ExoY-encoding P. aeruginosa, massive production of cGMP and cUMP was observed, with little production of cAMP. Purified ExoY was a highly effective nucleotidyl cyclase with the substrate preference GTP >> UTP ~ ATP > CTP. Fluorescence resonance energy transfer studies with methylanthraniloyl-substituted nucleotides corroborated the preference of ExoY for GTP. In contrast to ExoY, CyaA induced accumulation of cyclic nucleotides in the order cAMP > cCMP > cUMP in mammalian cells, and edema factor induced only cAMP- and cCMP accumulation.

Table 1 Comparison of ExoY with CyaA and EF

Conclusion

ExoY is a nucleotidyl cyclase with preference for GTP, and the substrate-specificity of ExoY is clearly different from that of CyaA and edema factor. Our data open the door for future studies aiming at the elucidation of the as yet unknown pathophysiological function of ExoY and which role cCMP, cGMP and cUMP play in this process.

References

  1. 1.

    Yahr TL, Vallis AJ, Hancock MK, Barbieri JT, Frank DW: ExoY, an adenylate cyclase secreted by the Pseudomonas aeruginosa type III system. Microbiol. 1998, 95: 13899-13904.

  2. 2.

    Vallis AJ, Finck-Barbançon V, Jahr TL, Frank DW: Biological effects of Pseudomonas aeruginosa type III secreted proteins on CHO cells. Infection and Immunity. 1999, 67: 2040-2044.

  3. 3.

    Göttle M, Dove S, Steindel P, Shen Y, Tang WJ, Geduhn J, König B, Seifert R: Molecular analysis of the interaction of Bordetella pertussis adenylyl cyclase with fluorescent nucleotides. Mol Pharmacol. 2007, 72: 526-535. 10.1124/mol.107.034413.

  4. 4.

    Taha HM, Schmidt J, Göttle M, Suryanarayana S, Shen Y, Tang WJ, Gille A, Geduhn J, König B, Dove S, Seifert R: Molecular analysis of the interaction of anthrax adenylyl cyclase toxin, edema factor, with 2’(3’)-O-(N-(methyl)anthraniloyl)- substituted purine and pyrimidine nucleotides. Mol Pharmacol. 2009, 75: 693-703. 10.1124/mol.108.052340.

Download references

Author information

Correspondence to Ulrike Voigt.

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Keywords

  • Nucleoside
  • Pseudomonas Aeruginosa
  • Pertussis
  • Adenylyl Cyclase
  • Fluorescence Resonance Energy Transfer