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A role for cGMP-dependent protein kinase II in AMPA receptor trafficking and synaptic plasticity
BMC Pharmacology volume 9, Article number: S44 (2009)
Trafficking of AMPA receptors (AMPARs) underlies the activity-dependent modification of synaptic strength and is regulated by specific interactions of AMPAR subunits with other proteins. We have reported (Serulle et al., 2007)  that the AMPAR subunit GluR1 binds the cGMP-dependent kinase type II (cGKII) adjacent to the kinase catalytic site, and that this interaction is increased by cGMP. In this complex, cGKII phosphorylates GluR1 at serine 845 (S845) leading to an increase of GluR1 on the plasma membrane. In neurons, cGMP is produced by soluble guanylate cyclase (sGC), which is activated by nitric oxide (NO), which is produced by nNOS under the control of the NMDA receptor.
To distinguish the mechanism, we have measured the rate of exogenous GluR1 endocytosis in cultured primary neurons, either the wild type or the S845A or S845D mutants (Figure 1). We find that the S845A mutant (which cannot be phosphorylated) is endocytosed at rate of the wild type, while S845D (phosphomimetic) is endocytosed at a lower rate. Also, cGMP treatment, which elevates the endogenous GluR1 plasma membrane levels (Figure 2A), reduced the rate of of GluR1 endocytosis (Figure 2B).
These data suggest that S845 phosphorylation increases the plasma membrane levels of GluR1 by reducing the rate of endocytosis.
Serulle Y, Zhang S, Ninan I, Puzzo D, McCarthy M, Khatri L, Arancio O, Ziff EB: A GluR1-cGKII interaction regulates AMPA receptor trafficking. Neuron. 2007, 56: 670-688. 10.1016/j.neuron.2007.09.016.
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Serulle, Y., Ninan, I., Puzzo, D. et al. A role for cGMP-dependent protein kinase II in AMPA receptor trafficking and synaptic plasticity. BMC Pharmacol 9, S44 (2009). https://doi.org/10.1186/1471-2210-9-S1-S44
- Nitric Oxide
- NMDA Receptor
- AMPA Receptor
- Guanylate Cyclase
- Synaptic Strength