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Evidence for two catalytic centers and Mn2+ as physiological cofactor of soluble guanylyl cyclase

  • Kerstin Yvonne Beste1Email author,
  • Johannes-Peter Stasch2,
  • Volkhard Kaever1 and
  • Roland Seifert1
BMC Pharmacology201111(Suppl 1):P7

https://doi.org/10.1186/1471-2210-11-S1-P7

Published: 1 August 2011

Keywords

Nitric OxideCyclic NucleotideCyclase ActivityCatalytic CenterPurine Nucleotide

Background

Soluble guanylyl cyclase (sGC) constitutes a family of enzymes that catalyses the cyclization of guanosine 5’-triphosphate (GTP) to guanosine 3’,5’-cyclic monophosphate (cGMP). The heterodimeric hemoprotein is activated by nitric oxide and mediates a wide range of physiological effects like regulation of blood pressure and neuronal cell development. sGC is an important target for the treatment of cardiovascular diseases.

In addition to the biosynthesis of cGMP, we recently showed that in vitro and in presence of Mn2+ ions sGC also generates the cyclic purine nucleotides adenosine 3’,5’-cyclic monophosphate (cAMP), inosine 3’,5’-cyclic monophosphate (cIMP), and xanthosine 3’,5’-cyclic monophosphate (cXMP)[1]. For all purine nucleotides, a second low-affinity site was described[1]. Moreover, sGC shows a pyrimidinylyl cyclase activity for uridine 3’,5’-cyclic monophosphate (cUMP) and cytosine 3’,5’-cyclic monophosphate (cCMP). In this case, no second binding site could be identified[1]. Lastly, no formation of the cyclic desoxyribonucleotide thymidine 3’,5’-cyclic monophosphate (cTMP) was detected[1].

Materials and methods

For in vitro assays and determination of Michaelis-Menten kinetic parameters highly purified recombinant sGC from rat (α1β1) was activated by sodium nitroprusside (100 µM). sGC (0.1-20 ng/tube) was incubated at 37°C with 3 mM MnCl2 or MgCl2 and various concentrations of nucleoside 5’-triphosphates (NTPs). Samples were stopped by heating at 95°C. Concentrations of cyclic nucleotides were determined by HPLC-MS/MS.

The in vivo assays were performed using transiently α1β1-transfected HEK 293 and endogenously sGC-expressing rat fetal lung fibroblast RFL-6 cells[2]. Cells were stimulated with SNP (100 µM) with or without phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX, 100 µM). After defined times medium was removed and cell metabolism was stopped by addition of organic solvent and by heating at 98°C. After centrifugation clear supernatant was taken for quantitation of cyclic nucleotides by HPLC-MS/MS.

Results

Analyzing in vitro substrate specificity in presence of Mg2+ revealed that cyclase activity of sGC is limited to purine nucleotides. Beside cGMP, cAMP, cIMP, and cXMP, but no cUMP and cCMP were generated. Additionally, in contrast to Mn2+ as cofactor, kinetics could be described completely using a model of a single binding site, suggesting that when Mg2+ ions are present only one high-affinity binding site is active.

However, studying transiently sGC-transfected HEK293 and endogenously sGC-expressing RFL-6 cells after SNP-stimulation we could detect a time-dependent accumulation of cGMP and cAMP as well as a generation of cCMP and cUMP. cXMP, cIMP and cTMP could not been detected. The addition of IBMX could only inhibit degradation of cGMP and cAMP while concentrations of cCMP and cUMP were only marginally influenced. These data indicate that Mn2+ rather than Mg2+ is the relevant divalent cation for sGC. Moreover, cCMP and cUMP appear to be hydrolysis-resistant in HEK293 and RFL-6 cells. Figure 1.
Figure 1
Figure 1

Model of two catalytic active binding sites in sGC

Conclusion

  1. 1.

    In the presence of Mg2+, sGC exhibits one catalytic center but in the presence of Mn2+, there are two catalytic centers.

     
  2. 2.

    Furthermore, in intact cells, NO not only stimulates cGMP production, but also cAMP, cCMP and cUMP synthesis.

     
  3. 3.

    These data indicate that in vivo, Mn2+ rather than Mg2+ may be the relevant divalent cation for sGC.

     

Declarations

Acknowledgements

We would like to thank Huige Li and Ulrich Förstermann for kindly providing RFL-6 cells.

Authors’ Affiliations

(1)
Institute of Pharmacology, Hannover Medical School, Hannover, Germany
(2)
Institute of Cardiovascular Research, Bayer HealthCare, Wuppertal, Germany

References

  1. Beste KY, Stasch JP, Kaever V, Seifert R: Nucleotidyl cyclase activity of guanylyl cyclases. Naunyn-Schmiedeberg´s Arch Pharmacol. 2010, 381 (Suppl 1): 27-Google Scholar
  2. Ishii K, Sheng H, Warner TD, Förstermann U, Murad F: A simple and sensitive bioassay method for detection of EDRF with RFL-6 rat lung fibroblasts. Am J Physiol. 1991, 261: H598-603.PubMedGoogle Scholar

Copyright

© Beste et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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