Skip to main content


  • Meeting abstract
  • Open Access

The effect of cetirizine, an H1 receptor antagonist, on bone modeling during orthodontic tooth movement in rats

  • 1,
  • 2, 3Email author,
  • 4,
  • 5,
  • 2 and
  • 1
BMC Pharmacology20099 (Suppl 2) :A65

  • Published:


  • Osteocalcin
  • Bone Turnover Marker
  • Cetirizine
  • Orthodontic Treatment
  • Tooth Movement


H1 receptor antagonists are widely used drugs for treatment of allergic conditions. While histamine is involved in bone remodelling [13], the aim of this study was to determine the effects of cetirizine, an H1 receptor antagonist, on bone modeling processes during orthodontic tooth movement.


We used three groups of Wistar rats: control group (n = 16), orthodontic appliance only group (n = 16) and cetirizine group (n = 16). Animals of the last two groups were fitted with a super-elastic closed coil spring appliance (F = 25 cN) between the upper first left molar and the upper incisors. Animals of the appliance only group were treated daily with saline and animals of the cetirizine group with 3 mg/kg of cetirizine, respectively. Tooth movement was measured weekly from day 0 to day 42. Animals of each group were sacrificed on day 42 and tissue samples were prepared for further analysis. Gene expression levels for bone turnover markers cathepsin K and osteocalcin were determined by means of RT-PCR. Alveolar bone volume, osteoblast and osteoclast volume were determined histomorphometrically.


Cetirizine decreased the amount of tooth movement from day 28 onwards (p < 0.01) and it also decreased osteoclast volume (p < 0.001). The increase in the alveolar bone volume was observed in the cetirizine group (p < 0.01) compared to the appliance only group. No significant difference was observed in osteoclast activity, osteoblast volume and osteoblast activity between the cetirizine and the appliance only groups.


Cetirizine influences bone modeling, mainly by inhibiting bone resorption. Therefore, H1 receptor antagonists therapy is supposed to interfere with orthodontic treatment.

Authors’ Affiliations

Department of Orthodontics, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia
Institute of Pharmacology and Experimental Toxicology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia
Ort-line, Institute for Orthodontics and General Dentistry, 1000 Ljubljana, Slovenia
Institute of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, 1000 Ljubljana, Slovenia
Department of Medical and Natural Sciences, College of Health Care, University of Primorska, 6000 Koper, Slovenia


  1. Seeman E: Structural basis of growth-related gain and age-related loss of bone strength. Rheumatology. 2008, 47 (Suppl 4): iv2-iv8. 10.1093/rheumatology/ken177.PubMed CentralPubMedGoogle Scholar
  2. Lesclous P, Schramm F, Gallina S, Baroukh B, Guez D, Saffar JL: Histamine mediates osteoclastic resorption only during the acute phase of bone loss in ovariectomized rats. Exp Physiol. 2006, 9: 561-570. 10.1113/expphysiol.2006.033217.View ArticleGoogle Scholar
  3. Deyama Y, Kikuiri T, Ohnishi G, Feng YG, Takeyama S, Hatta M, Yoshimura Y, Suzuki K: Histamine stimulates production of osteoclast differentiation factor/receptor activator of nuclear factor-κB ligand by osteoblasts. Biochem Biophys Res Commun. 2002, 298: 240-246. 10.1016/S0006-291X(02)02440-3.View ArticlePubMedGoogle Scholar


© Meh et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.