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Role of PKG II in osteoblast mechanotransduction
BMC Pharmacology volume 9, Article number: S32 (2009)
Mechanical stress is a primary determinant of bone growth and remodeling: weight bearing and locomotion stimulate interstitial fluid flow through the bone canalicular system, and the resultant shear stress is a major mechanism whereby mechanical forces stimulate bone growth . In response to fluid shear stress and other types of mechanical stimulation, osteoblasts produce large amounts of NO, and genetic and pharmacologic studies indicate an important role of NO in osteoblast biology, but little is known about signaling downstream of NO in osteoblasts [1, 2].
In primary human osteoblasts and murine MC3T3-E1 cells, we found that fluid shear stress induced rapid expression of c-fos, fra-1, fra-2, and fosB/ΔfosB mRNAs; these genes encode transcriptional regulators important for osteoblast proliferation and differentiation, as demonstrated by severe osteosclerotic or osteoporotic phenotypes of mice that over-express or lack these proteins, respectively. Fluid shear stress increased osteoblast nitric oxide (NO) synthesis, leading to increased cGMP production and activation of cGMP-dependent protein kinases (PKG), as demonstrated by phosphorylation of the PKG I/II substrate VASP. Pharmacological inhibition of the NO/cGMP/PKG signaling pathway blocked shear-induced expression of all four fos family genes. Induction of these genes required signaling through MEK/Erk, and Erk activation was NO/cGMP/PKG-dependent. Treating cells with a membrane-permeable cGMP analog partly mimicked the effects of fluid shear stress on Erk activity and fos family gene expression, and it appears that cGMP co-operates with increased intracellular calcium in shear-stressed osteoblasts. In cells transfected with siRNAs specific for membrane-bound PKG II, shear- and cGMP-induced Erk activation and fos family gene expression was nearly abolished and could be restored by transducing cells with a virus encoding an siRNA-resistant form of PKG II; in contrast, siRNA-mediated repression of the more abundant cytosolic PKG I isoform was without effect.
Thus, we report a novel function for PKG II in osteoblast mechanotransduction, and propose a model whereby NO/cGMP/PKG II-mediated Erk activation and induction of c-fos, fra-1, fra-2, and fosB/ΔfosB play a key role in the osteoblast anabolic response to mechanical stimulation. Defective PKG II regulation of fos family transcription factors in osteoblasts may contribute to the developmental bone defects observed in PKG II-deficient rodents .
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Aguirre J, Buttery L, O'Shaughnessy M, Afzal F, de Marticorena IF, Hukkanen M, Huang P, Maclntyre I, Polak J: Endothelial nitric oxide synthase gene-deficient mice demonstrate marked retardation in postnatal bone formation, reduced bone volume, and defects in osteoblast maturation and activity. Am J Pathol. 2001, 158: 247-257.
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Rangaswami, H., Zhuang, N.M.S., Chen, Y. et al. Role of PKG II in osteoblast mechanotransduction. BMC Pharmacol 9, S32 (2009). https://doi.org/10.1186/1471-2210-9-S1-S32
- Nitric Oxide
- Fluid Shear Stress
- Primary Human Osteoblast
- Interstitial Fluid Flow
- cGMP Analog