Blockade of CNG channels abrogates urethral relaxation induced by soluble guanylate cyclase activation
© Triguero et al; licensee BioMed Central Ltd. 2009
Published: 11 August 2009
This effect was stereoselective since the isomer D-cis-diltiazem did not have any effect on urethral relaxations. Similarly, L-cis-diltiazem (50 μM) showed a complete blockade of the relaxation induced by the addition of YC-1 (50 μM), an specific activator of the soluble guanylate cyclase (sGC) (Figure 1B), thus reinforcing that L-cis-diltiazem would be acting as specific inhibitor of CNG channels in this tissue.
The presence of these channels in urethral tissue was tested by carrying out conventional RT-PCR studies using retina as a positive control. A band of the predicted size showing after sequencing, a 99% identity with the rod-type CNGA1, together to a second band with a similar size to that ofthe CNGB1 fragment, were obtained. These results suggest that they are heteromeric retina-like CNG channels.
This work was supported by a grant (BFU2006-15135-C02-01) from the Ministerio de Educación y Ciencia of Spain.
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