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  • Open Access

The role of cGMP-cGKI-signaling for duodenal bicarbonate secretion

  • 1Email author,
  • 1,
  • 1,
  • 1,
  • 2,
  • 3,
  • 3,
  • 4 and
  • 1, 5
BMC Pharmacology20099(Suppl 1):P67

Published: 11 August 2009


  • Gastric Acid
  • Duodenal Mucosa
  • Basal Secretion
  • Chronic Anemia
  • Bicarbonate Secretion


The duodenal mucosa protects itself from gastric acid injury by the secretion of bicarbonate from epithelial cells. We determined a possible role of the cGMP-cGKI pathway for the duodenal bicarbonate secretion by studying conventional cGKI knockouts (cGKI-KOs) and rescue mice (RM) that express either the cGKIα or Iβ isoform in SM22α positive smooth muscle cells [1].


The basal secretion rate of bicarbonate was strongly reduced in the different gene-targeted cGKI mice. Moreover, the H+ induced bicarbonate secretion was significantly increased in controls but nearly absent in all cGKI mutants, whereas invasive pH measurements in fasted animals demonstrated that the gastric acid production of all genotypes was similar. The dysfunction of the duodenal bicarbonate secretion of RM and cGKI-KO animals was associated with severe gastrointestinal bleedings, which were caused by the age-dependent aggravation of an epithelial ulceration that localized to the papilla Vateri.


The analysis of cGKI-KO and RM indicates that a cGMP-cGKI-dependent pathway is present in non-smooth muscle cells of the duodenum that is involved in the basal and acid-induced secretion of bicarbonate. The inability to secrete adequate amounts of bicarbonate ultimately leads to duodenal ulceration. We postulate that the continuous blood loss accounts for the chronic anemia of cGKI mutant mice and causes the premature death of the cGKI-KOs and RM.



We greatly thank Teodora Ruttner for her skilled technical support.

Authors’ Affiliations

For 923 at Institut für Pharmakologie und Toxikologie, TU München, Germany
Department of Internal Medicine II, Klinikum Rechts der Isar, TU München, Germany
Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Germany
Department of Pharmacology and Toxicology, Universität Regensburg, Germany
Institut für Pharmakologie und Toxikologie, TU München, Germany


  1. Weber S, Bernhard D, Lukowski R, Weinmeister P, Wörner R, Wegener JW, Valtcheva N, Feil S, Schlossmann J, Hofmann F, Feil R: Rescue of cGMP kinase I knockout mice by smooth muscle specific expression of either isozyme. Circ Res. 2007, 101: 1096-103. 10.1161/CIRCRESAHA.107.154351.View ArticlePubMedGoogle Scholar


© Spiessberger et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.