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Open Access

Effect of L-carnitine supplementation on the sGC/cGMP pathway in vascular relaxing responses from exercised rats

  • Fernanda BM Priviero1Email author,
  • Julio A Rojas-Moscoso2,
  • Alexandre S Silva1,
  • Edson Antunes2 and
  • Angelina Zanesco1
BMC Pharmacology20099(Suppl 1):P54

https://doi.org/10.1186/1471-2210-9-S1-P54

Published: 11 August 2009

Background

It has been largely documented the beneficial effects of physical training on the endothelium-derived relaxing response by increasing nitric oxide production and/or its bioavailability to the smooth muscle [1]. L-carnitine (L-Car) has been used as important supplement to regulate body composition associated with lipid metabolism. However, no studies exist investigating the effect of L-Car intake associated with dynamic exercise on the NO-independent sGC/cGMP pathway in the vascular responsiveness in rats. Thus, the aim of this work was to investigate the effect of L-Car supplementation on the NO-independent sGC/cGMP pathway in aortic and mesenteric rings in trained rats.

Methods

Male Wistar rats (344 ± 6 g) were divided into three groups: sedentary (SD), sedentary supplemented (SDS) and trained supplemented (TRS). Animals were trained in a treadmill with an intensity of 70–80% of maximal oxygen consumption, in sessions of 60 minutes, 5 days a week. Run training (RT) was performed simultaneously to L-Car intake (0.2 g/kg daily, given in the drinking water) for 4 weeks. Concentration-response curves were obtained for sodium nitroprusside (SNP) in isolated aortic and mesenteric rings. Plasma SOD activity and catalase levels were measured.

Results

Oral supplementation with L-Car associated with run training provoked a significant reduction in body weight gain (368 ± 13 g) as compared to sedentary groups (SD: 432 ± 9 and SDS: 433 ± 15 g). In aortic rings, the potency (pEC50) for SNP was increased in TRS group as compared to sedentary groups. In mesenteric rings, supplementation with L-Car provokes an increase in the endothelium-independent relaxing response at the pEC50 level in sedentary and trained animals (SDS and TRS groups). No changes were seen in the maximal responses to SNP in both preparations. Data are summarized in Table 1. Neither the exercise program nor the chronic supplementation with L-Car affected the plasma levels of catalase (44 ± 14 μM, 69 ± 8 μM and 72 ± 25 μM, for SD, SDS and TRS groups, respectively) and SOD activity (9 ± 4 U/ml, 12 ± 3 U/ml and 11 ± 2 U/ml, for SD, SDS and TRS groups, respectively).
Table 1

Potency (pEC50) and maximal responses (EMAX) for sodium nitroprusside (SNP) in mesenteric and aortic rings from sedentary and exercised rats treated with L-Car supplementation for 4 weeks.

Groups

Sodium Nitroprusside

 

Mesenteric

Aorta

 

pEC50

EMAX

pEC50

EMAX

SD

8.31 ± 0.06

103 ± 2

7.90 ± 0.13

109 ± 2

SDS

8.81 ± 0.04*

103 ± 3

7.99 ± 0.13

95 ± 3

TRS

8.58 ± 0.03*

109 ± 4

8.61 ± 0.07*

114 ± 5

Data are means SEM for 4–6 experiments. *, different from sedentary group.

Conclusion

In conclusion, L-Car supplementation associated with physical exercise training was effective to reduce body weight gain in rats. Furthermore, an increase in the relaxing response for SNP in aortic and mesenteric rings from exercise rats treated with L-Car supplementation. These findings suggest that physical exercise-induced shear stress could affect directly the sGC/cGMP signaling pathway in vascular tissues.

Declarations

Acknowledgements

Financial Support: FAPESP

Authors’ Affiliations

(1)
Department of Physical Education, São Paulo State University, UNESP
(2)
Department of Pharmacology, State University of Campinas, UNICAMP

References

  1. Zanesco A, Antunes E: Effects of exercise training on the cardiovascular system: Pharmacological approaches. Pharmacol Ther. 2007, 114: 307-17. 10.1016/j.pharmthera.2007.03.010.View ArticlePubMedGoogle Scholar

Copyright

© Priviero et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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