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Open Access

Bioactivation of nitroglycerin by the East Asian variant of aldehyde dehydrogenase-2

  • Matteo Beretta1Email author,
  • Antonius CF Gorren1,
  • M Verena Wenzl1,
  • Robert Weis2,
  • Michael Russwurm3,
  • Doris Koesling3,
  • Kurt Schmidt1 and
  • Bernd Mayer1
BMC Pharmacology20099(Suppl 1):P3

https://doi.org/10.1186/1471-2210-9-S1-P3

Published: 11 August 2009

Background

The East Asian variant of mitochondrial aldehyde dehydrogenase (ALDH2) exhibits significantly reduced dehydrogenase, esterase and nitroglycerin (GTN) reductase activities [1]. The small molecule Alda-1 was reported to partly restore low acetaldehyde dehydrogenase activity of this variant [2]. In the present study we compared the wild type enzyme (ALDH2*1) with the East Asian variant (ALDH2*2) regarding GTN bioactivation and effects of Alda-1.

Results

Alda-1 increased acetaldehyde oxidation by ALDH2*1 and ALDH2*2 about 2- and 15-fold, respectively. The effects of this compound on the esterase activities of both enzymes were identical to that of NAD (6- and 10-fold stimulation with ALDH2*1 and ALDH2*2, respectively), but in the presence of the nicotinamide Alda-1 stimulated the esterase activity of ALDH2*1 only 1.7-fold, whereas the ALDH2*2-catalyzed reaction was increased 73-fold.

ALDH2*1 exhibited a greater affinity for GTN than ALDH2*2 (with Km values of 7.6 ± 1.4 and 54 ± 6 μM, respectively) as well as a 7-fold higher maximal GTN denitration activity. However, bioactivation of the nitrate, measured as soluble guanylate cyclase (sGC) activation, was much more pronounced (8.8 ± 0.2 and 18.2 ± 0.9 μmol cGMP × min-1 × mg-1 in the presence of 100 μg/100 μL ALDH2 and 100 μM GTN). Alda-1 caused 30–70% inhibition of GTN denitration by ALDH2*1 but had no effect on the ALDH2*2-catalyzed reaction and did not affect GTN-induced sGC activation in the presence of either variant.

Conclusion

The present results indicate that Alda-1 stimulates established ALDH2 activities by improving NAD binding but does not restore impaired GTN bioactivation by the East Asian variant. In addition, our data revealed an unexpected discrepancy between GTN reductase activity and sGC activation, suggesting that GTN denitration and bioactivation reflect independent reactions catalyzed by ALDH2.

Declarations

Acknowledgements

Supported by Fonds zur Förderung der Wissenschaftlichen Forschung in Austria (W901 DK Molecular Enzymology and P20669) and Deutsche Forschungsgemeinschaft (KO1157/4-1).

Authors’ Affiliations

(1)
Department of Pharmacology and Toxicology, Karl-Franzens-Universität Graz
(2)
Department of Pharmaceutical Chemistry, Karl-Franzens-Universität Graz
(3)
Department of Pharmacology and Toxicology, Ruhr-Universität Bochum

References

  1. Larson HN, Zhou J, Chen Z, Stamler JS, Weiner H, Hurley TD: Structural and functional consequences of coenzyme binding to the inactive Asian variant of mitochondrial aldehyde dehydrogenase. J Biol Chem. 2007, 282: 12940-12950. 10.1074/jbc.M607959200.PubMed CentralView ArticlePubMedGoogle Scholar
  2. Chen CH, Budas GR, Churchill EN, Disatnik MH, Hurley TD, Mochly-Rosen D: Activation of aldehyde dehydrogenase-2 reduces ischemic damage to the heart. Science. 2008, 321: 1493-1495. 10.1126/science.1158554.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Beretta et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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