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Open Access

NO-sensitive guanylyl cyclase β1 subunit interacts with chromosomes during mitosis: novel role in the regulation of chromatin condensation

  • Paula Pifarré1Email author,
  • María Antonia Baltrons1,
  • Verónca Davalos2,
  • Simon SchwartzJr2 and
  • Agustina García1
Contributed equally
BMC Pharmacology20077(Suppl 1):S43

https://doi.org/10.1186/1471-2210-7-S1-S43

Published: 25 July 2007

Background

NO-sensitive guanylyl cyclase (GCNO), the major NO target, exists as an obligate heterodimer of one α and one β subunit. Two types of each subunit have been cloned (α1–2, β1–2), but only β1 and α subunit expression has been reported in the CNS. In this tissue, in situ hybridization studies have shown that β1 is more widespread than α subunits and in some areas is the only GCNO subunit expressed [1]. Since β1/β1 homodimers are catalytically inactive the possibility of β1 having functions other than GCNO activity has been suggested. GCNO is predominantly cytosolic, however recent studies suggest that it can associate to membranes and other intracellular structures including nuclei [2, 3].

Results

In the course of our studies on the cellular and sub-cellular distribution of GCNO subunits in CNS glial cells we have found that the β1 subunit is localized in the cytoplasm and the nucleus in cells that also express α subunits and present GCNO activity (astrocytes), as well as in cells devoid of α subunits and GCNO activity (microglia). In both cases GCNO β1 associates peripherally to chromosomes in all phases of mitosis and appears to regulate mitotic chromatin condensation independent of cGMP formation. Moreover, silencing by siRNA increases the percentage of cells in the S phase of the cell cycle and enhances proliferation.

Conclusion

The GCNO β1 subunit associates to chromosomes during mitosis and regulates chromatin condensation and cell cycle progression decreasing cell proliferation. This actions of GCNO β1 are independent of NO-dependent cGMP formation.

Notes

Declarations

Acknowledgements

This work has been supported by Ministerio de Educación y Ciencia, Spain, SAF2004-01717; Fellowship FPU 2000 to P. Pifarré.

Authors’ Affiliations

(1)
Institute of Biotechnology and Biomedicine and Department of Biochemistry and Molecular Biology, Autonomous University of Barcelona
(2)
Molecular oncology Program, Molecular Oncology and Aging Group, Vall d'Hebron Hospital Research Institute

References

  1. Pifarre P, Garcia A, Mengod G: Species differences in the localization of soluble guanylyl cyclase subunits in monkey and rat brain. J Comp Neurol. 2007, 500: 942-947. 10.1002/cne.21241.View ArticlePubMedGoogle Scholar
  2. Nedvetsky PI, Sessa WC, Schmidt HH: There's NO binding like NOS binding: protein-protein interactions in NO/cGMP signalling. Proc Natl Acad Sci USA. 2002, 99: 16510-16512. 10.1073/pnas.262701999.PubMed CentralView ArticlePubMedGoogle Scholar
  3. Gobeil F, Zhu T, Brault S, Geha A, Vazquez-Tello A, Fortier A, Barbaz D, Checchin D, Hou X, Nader M: Nitric oxide signaling via nuclearized endothelial nitric-oxide synthase modulates expression of the immediate early genes iNOS and mPGES-1. J Biol Chem. 2006, 281: 16058-16067. 10.1074/jbc.M602219200.View ArticlePubMedGoogle Scholar

Copyright

© Pifarré et al; licensee BioMed Central Ltd. 2007

This article is published under license to BioMed Central Ltd.

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