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Hyper-contractility and impaired cGMP signaling in the BKCa channel deletion model of erectile dysfunction
BMC Pharmacology volume 7, Article number: P65 (2007)
Erectile dysfunction (ED) is frequently elicited by a multiplicity of pathogenic factors, predominantly by impaired formation of and responsiveness to nitric oxide (NO) and the downstream effectors soluble guanylate cyclase (sGC) and cGMP-dependent protein kinase I (PKGI). In smooth muscle, one important target of PKGI is the large conductance, calcium-sensitive potassium (BKCa) channel, which upon activation hyperpolarizes the smooth muscle cell membrane, causing relaxation. In our earlier report , we demonstrated that ablation of the gene, encoding for the pore-forming α subunit of the BKCa channel in mice (Slo-/-) induced an increase of corpus cavernosum smooth muscle (CCSM) force oscillations, led to reduced nerve-evoked relaxations and ED. In our current work, we used this ED model to explore the role of the BKCa channel in the NO/cGMP pathway. Electrical field stimulation (EFS)-induced contractions of CCSM strips from Slo-/- mice demonstrated a 53% increase that could be reduced by sildenafil similar to levels observed in strips from wild-type (Slo+/+) mice. In Slo-/- strips precontracted with phenylephrine (PE), SNP and sildenafil induced relaxations, which were diminished by 10% and 7% over Slo+/+, respectively. Neither SNP nor sildenafil was able to reduce the enhanced force oscillations, which were induced by the loss of BKCa channel function. Yet, these oscillations could be completely eliminated by blocking L-type voltage-dependent calcium channels (VDCCs). The latter results indicate that loss of BKCa channel leads to ED and hyper-contractility likely due to instability of membrane potential which activates VDCCs. Moreover, since the relaxing effects of SNP and sildenafil were reduced in Slo-/-, the ED phenotype in our BKCa channel deletion model could also be the consequence of an impaired NO/cGMP signaling pathway.
Werner ME, Zvara P, Meredith AL, Aldrich RW, Nelson MT: Erectile dysfunction in mice lacking the large conductance calcium-activated (BK) channel. J Physiol. 2005, 567: 545-556/Pkt.2. 10.1113/jphysiol.2005.093823.
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Werner, M.E., Meredith, A.L., Aldrich, R.W. et al. Hyper-contractility and impaired cGMP signaling in the BKCa channel deletion model of erectile dysfunction. BMC Pharmacol 7, P65 (2007). https://doi.org/10.1186/1471-2210-7-S1-P65
- Nitric Oxide
- Erectile Dysfunction
- Guanylate Cyclase
- Force Oscillation