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Properties of the transient outward, ultra-rapid delayed rectifier and acetylcholine-sensitive potassium currents in isolated atrial myocytes from dogs: sinus rhythm and tachypaced model of permanent atrial fibrillation

  • Zsófia Kohajda1,
  • Attila Kristóf1,
  • Péter P Kovács1,
  • Claudia Corici1,
  • László Virág1,
  • Viktor Juhász1,
  • Zoltán Husti1,
  • István Baczkó1,
  • András Varró1 and
  • Norbert Jost1Email author
BMC Pharmacology201111(Suppl 2):A60

https://doi.org/10.1186/1471-2210-11-S2-A60

Published: 5 September 2011

Background

Atrial fibrillation (AF) is a common and severe arrhythmia, which largely affects quality of life. State-of-the-art treatment of AF still relies heavily on pharmacological modalities. Therefore, the aim of the present study was to investigate and compare the properties of three repolarizing currents which contribute to AF-induced remodeling, i.e. the transient outward (Ito), ultra-rapid delayed rectifier (IKur) and acetylcholine-sensitive potassium currents (IK,ACh) in isolated atrial myocytes obtained from normal (SR) and tachypaced model of permanent atrial fibrillation (ATR) dogs.

Methods

The tachypaced atrial fibrillation model was performed in dogs. Transmembrane ionic currents were investigated by applying the whole-cell patch clamp technique at 37°C, and ECG was recorded in conscious dogs.

Results

In all atrial canine myocytes, we have identified an Ito current sensitive to 4-aminopyridine (4-AP; 3 mM). The current inactivation was best fitted by two exponentials. The Ito current was slightly downregulated in ATR cells when compared with that recorded in SR cells. The IKur current, measured as sustained current (Isus), was upregulated in ATR dogs. However, the selective IKur blocker 4-AP (50 µM) did not block either Isus or IKur „like tail” currents, which questions the reliability of these results. IK,ACh was activated by the cholinergic agonist carbachol (CCh; 2 µM). In SR, CCh activated a large current either at inward or outward directions. The selective IK,ACh blocker tertiapin (10 nM) blocked the CCh-induced current by 57%. In atrial myocytes from ATR dogs we could measure the presence of a constitutively active IK,ACh, which could be blocked by 26 % with 10 nM tertiapin. However, in ATR atrial myocytes, CCh in addition could also activate a significant ligand-dependent and tertiapine-sensitive IK,ACh current. Tertiapin effectively prevented burst-induced AF in conscious ATR dogs.

Conclusions

The presence of the constitutively activated IK,ACh in atrial myocytes from ATR dogs shows that electrical remodeling developed in our model; this was further supported by the inducibility of AF by rapid atrial bursts in these dogs. The IK,ACh current (both ligand-dependent and constitutively active currents) seems to play a significant role in the canine atrial electrical remodelling, and may be a promising drug target for suppressing AF.

Declarations

Acknowledgements

This work was funded by grants from OTKA (CNK-77855, K-82079) and the National Development Agency (TÁMOP-4.2.1/B-09/1/KONV-2010-0005).

Authors’ Affiliations

(1)
Department of Pharmacology and Pharmacotherapy, University of Szeged; Division of Cardiovascular Pharmacology, Hungarian Academy of Sciences

Copyright

© Kohajda et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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