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Open Access

Neurodegeneration and histochemical plasticity in the rat subiculum after kainic acid-induced epilepsy

  • Meinrad Drexel1Email author,
  • Adrian P Preidt1,
  • Elke Kirchmair1 and
  • Günther Sperk1
BMC Pharmacology201111(Suppl 2):A17

https://doi.org/10.1186/1471-2210-11-S2-A17

Published: 5 September 2011

Background

The subiculum, the main output region of the hippocampus, remains largely preserved in human temporal lobe epilepsy (TLE) and therefore may be importantly involved in the generation of epileptic activity arising from the hippocampal region. Our goal was to characterize histopathological and neurochemical changes in the rat subiculum using the kainic acid (KA) model of TLE and to correlate these alterations with data from EEG-recordings.

Methods

Rats (n = 35) were implanted with biopotential transmitters (EA-F20, DSI) for continuous EEG/video-monitoring. One week later, they were injected with KA (i.p., 10 mg/kg) and developed seizures and an initial status epilepticus (SE). Rats were killed 1, 8, 30 or 90 days after the initial SE and brain histopathology was investigated using immunohistochemistry and in situ hybridization for neuropeptides and calcium-binding proteins as markers for different neuronal subpopulations.

Results

Rats developed spontaneous seizures 3 to 36 days (15 ± 1.5 d) after the initial SE. Neurodegeneration and reactive gliosis were more pronounced in the proximal than in the distal part of the subiculum. The number of parvalbumin (PV)-ir GABAergic interneurons was significantly reduced in the pyramidal cell layer of the subiculum already 24 hrs after KA injection. The decrease in the number of PV-positive neurons in the subiculum correlated with the number of spontaneous seizures subsequently experienced by the rats. Increased (or even de novo) expression of neurokinin B (NKB) and NPY mRNA was observed in pyramidal neurons of the subiculum, and fiber labeling for NKB and NPY was increased at late intervals after SE.

Conclusions

Early degeneration of PV-ir GABAergic basket- and axo-axonic cells may result in decreased inhibition of pyramidal neurons and affects the numbers of spontaneous seizures occurring later. Since NKB has an excitatory action (activating phospholipase C) on NK3 receptors, expression of the peptide in principal neurons of the subiculum may contribute to the generation of epileptic seizures. On the other hand, ectopic expression and subsequent release of NPY from axon terminals of pyramidal neurons may inhibit glutamate release by activating presynaptically located Y2 receptors and thus exert an anticonvulsive action.

Declarations

Acknowledgements

Supported by the Austrian Research Funds (P 19464) and the European Union Grant FP6 EPICURE (LSH-CT-2006-037315).

Authors’ Affiliations

(1)
Department of Pharmacology, Medical University Innsbruck

Copyright

© Drexel et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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