Volume 11 Supplement 1

5th International Conference on cGMP: Generators, Effectors and Therapeutic Implications

Open Access

Conjoined NO-sensitive guanylyl cyclases

  • Sönke Behrends1Email author,
  • Mareike Busker1,
  • Nadine Haase1,
  • Tobias Haase1,
  • Jan Krähling1 and
  • Monika Linnenbaum1
BMC Pharmacology201111(Suppl 1):P6

https://doi.org/10.1186/1471-2210-11-S1-P6

Published: 1 August 2011

Background

NO-sensitive guanylyl cyclases (NO-GC’s) that catalyze the reaction of GTP to the second messenger molecule cGMP are heterodimeric enzymes consisting of an α and a β1 subunit. The two prevalent isoforms in humans (α11, α21) are time-tested targets for drugs that release nitric oxide (NO) and new compounds that either sensitize the enzyme for activation by NO or activate the enzyme independently of NO. Homologous NO-GC subunits consist of an amino-terminal HNOX domain followed by a PAS- and a coiled coil domain and the carboxy-terminal catalytic domain.

Results

Measurement of FRET between fluorescent proteins tagged to the amino- and carboxy-terminus of the subunits indicated close proximity between the amino-terminal HNOX domains and the carboxy-terminal catalytic domains of the enzyme. On the basis of these results we constructed conjoined NO-GC’s by fusion of the α amino-terminus to the β1 carboxy-terminus leading to a monomeric enzyme complex. Surprisingly but in accordance with the FRET results these conjoined NO-GC’s (β1α1, β1α2) showed specific enzyme activity and stimulation by NO and various modulators of GC activity.

Conclusion

These obligate enzyme variants faithfully reproduced the pharmacological properties of the heterodimeric enzymes including an isoform specific differential activation of the NO-independent drug cinaciguat. Novel applications of conjoined NO-GC’s including adenoviral gene transfer will be discussed.

Authors’ Affiliations

(1)
Institut für Pharmakologie, Toxikologie und Klinische Pharmazie, TU Braunschweig

Copyright

© Behrends et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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