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The mechanism of CNP-induced negative inotropic and positive lusitropic responses are dependent on SERCA activity in failing rat ventricle
BMC Pharmacology volume 11, Article number: P49 (2011)
Natriuretic peptides increase in heart failure. C-type natriuretic peptide (CNP) generates cyclic 3’,5’ guanosine monophosphate (cGMP) by activating the NPR-B receptor in cardiomyocytes. There are studies showing CNP-induced negative inotropic and positive lusitropic responses in normal hearts, but less is known about the effects of CNP in failing hearts.
We investigated the functional effects of CNP in heart failure and suggested that increased activation of SERCA is the main mechanism of CNP-induced negative inotropic and positive lusitropic responses. Increased SERCA activity could cause a faster Ca2+ removal from cytosol and thus less Ca2+ available for the myofilaments during contraction. Cyclic GMP levels, contraction and relaxation, Ca2+ transients, troponin I (TnI) and phospholamban (PLB) phosphorylation were measured in left ventricular muscle strips or cardiomyocytes from Wistar rats with heart failure 6 weeks after myocardial infarction. CNP increased cGMP levels and evoked negative inotropic and positive lusitropic responses concentration-dependently. TnI and PLB phosphorylation also increased in the presence of CNP. The functional responses to CNP were reduced in the presence of a PKG-blocker/cGMP-analogue (Rp-8-Br-Pet-cGMPs), demonstrating activation of the PKG pathway. In the presence of CNP, Ca2+ transient amplitude and Ca2+ extrusion rate were increased. CNP elicited both negative inotropic and positive lusitropic responses in the presence of the L-type Ca2+ channel activator BAY K 8644, whereas in the presence of full activation of the cAMP system by isoproterenol these responses were not seen. This indicates that the downstream targets causing functional responses to CNP were already activated in the presence of isoproterenol. All these results could be explained by an increased SERCA activity and a reduced myofibrillar sensitivity to Ca2+ in the presence of CNP, due to increased phosphorylation of PLB and TnI, respectively. An obligatory role of SERCA activation was revealed in a mouse model with cardiomyocyte-specific excision of the SR Ca2+-ATPase gene (SERCA-KO). The functional responses to CNP were abolished in 8-week SERCA-KO mice compared to 4-week SERCA-KO mice still possessing some SERCA activity.
The functional responses to CNP are mediated through the PKG pathway. Activation of SERCA thus seems to be the major and indispensable mechanism of CNP-induced functional responses in failing hearts.
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Moltzau, L.R., Aronsen, J.M., Meier, S. et al. The mechanism of CNP-induced negative inotropic and positive lusitropic responses are dependent on SERCA activity in failing rat ventricle. BMC Pharmacol 11 (Suppl 1), P49 (2011). https://doi.org/10.1186/1471-2210-11-S1-P49
- Functional Response
- Extrusion Rate
- ATPase Gene
- SERCA Activity