Skip to main content

Advertisement

We’d like to understand how you use our websites in order to improve them. Register your interest.

PKA: assembly of dynamic macromolecular signaling

Although we have learned a great deal about the protein kinase superfamily from our structure/function studies of the free PKA catalytic (C) subunit and the free cAMP-bound regulatory (R) subunits, we did not understand how the C-subunit was inhibited in the holoenzyme complexes, nor did we understand at the molecular level how the holoenzymes were activated by cAMP. There are four functionally non-redundant R-subunits (RIα, RIβ, RIIα, and RIIβ) and each is assembled as a dimer. The N-terminal dimerization/docking (D/D) is fllowed by a flexible linker and two tandem camp binding domains. The D/D domain also serves as the docking site for A Kinase Anchoring Proteins (AKAPs) which serve as scaffolds that target the PKA holoenzyme to specific sites in the cell in close proximity to specific substrates. Isoform diversity of the R-subunits is a major mechanism for achieving specificity in PKA signaling. We describe here, for the first time, how PKA is assembled into diverse tetrameric holoenzymes and this allows us to begin to fully appreciate the role of the regulatory subunits in creating novel tetramers that are then recruited in unique ways to supramolecular complexes. How these scaffolds are then assembled as part of polyvalent PKA signaling scaffolds is our next challenge. By showing how the RII subunit is anchored to a PDZ domain through a dual specific AKAP, DAKAP2, we are beginning to understand how larger scaffolds are assembled and anchored to the C-termini of ion transporters. We are also exploring PKA signaling scaffolds at the mitochondria.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Susan S Taylor.

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Taylor, S.S., Ilouz, R., Darshi, M. et al. PKA: assembly of dynamic macromolecular signaling. BMC Pharmacol 11, O12 (2011). https://doi.org/10.1186/1471-2210-11-S1-O12

Download citation

Keywords

  • Protein Kinase
  • Regulatory Subunit
  • Docking Site
  • Supramolecular Complex
  • Anchor Protein