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Luminescence resonance energy transfer-based intramolecular distance measurements in leucine transporter from Aquifex aeolicus

Background

Solute carrier 6 (SLC6) membrane proteins are integral membrane proteins and of particular pharmacological interest because they are targets of many clinically important drugs. These SLC6 proteins play crucial roles ranging from nutrient uptake to neurotransmitter clearance. A leucine transporter LeuTAa from Aquifex aeolicus has been recognized as a bacterial orthologue of mammalian SLC6 family proteins. LeuTAa has been crystallized and its structure was resolved to high resolution. With respect to its kinship to other SLC6 transporters, though with low sequence identity (~20–25%), there are crucial regions in transmembrane segments 1, 3, 6 and 8 where conservation reaches ~50%. For this very reason LeuTAa provides a good structural paradigm to study homology models of SLC6 family members and learn more about the structure/function relationship in mammalian transporters.

Methods and results

In order to test proposed models, we initiated a study to measure intramolecular distance changes associated with the dynamic process of substrate transport. We employed luminescence resonance energy transfer (LRET) to measure the changes in intramolecular distances. For LRET-based measurements we have introduced LBT (lanthanide binding tags) to accommodate terbium, as the donor element, along with cysteines, where acceptor fluorophores are attached, at selected positions in LeuTAa. After expression and purification of these mutants, we obtained the first distances at atomic resolution.

Conclusions

Taken together our LRET measurements can help us to validate or propose a dynamic substrate transport model for LeuTAa. Our future plan focusses on the establishment of functionality assays for screening of functional LeuTAa mutants along with their LRET measurements.

Author information

Correspondence to Harald Sitte.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Keywords

  • Substrate Transport
  • SLC6 Family
  • SLC6 Transporter
  • Intramolecular Distance
  • Acceptor Fluorophores