Volume 10 Supplement 1

16th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access

Interaction of manganese with striatal dopamine turnover in human alpha-synuclein transgenic mice

  • Tamara M Peneder1,
  • Michael Berger1,
  • Petra Scholze1,
  • Georg Heinze2,
  • Johanna Bertl2,
  • Jan Bauer1,
  • Oleh Hornykiewicz1,
  • Eric K Richfield3 and
  • Christian Pifl1Email author
BMC Pharmacology201010(Suppl 1):A16

https://doi.org/10.1186/1471-2210-10-S1-A16

Published: 16 November 2010

Background

It is thought that the interaction of genetic and environmental factors is an important risk factor for Parkinson’s disease (PD). α-Synuclein (α-syn) is a protein of special interest in PD because mutations in α-syn (A53T or A30P or E46K) lead to PD. Manganese (Mn) is a heavy metal known to cause parkinsonian symptoms. Therefore, we investigate the effect of manganese (Mn) on human α-syn-expressing mice.

Materials and methods

C57/Bl6 mice expressing either human α-syn or the A53T/A30P doubly mutated human α-syn under the tyrosine hydroxylase promoter and nontransgenic sister mice were exposed at the age of 4 month to either MnCl2 (1%) enriched or control food. Locomotor activity was quantified every 2 months using automated activity chambers. Mice were sacrificed at the age of 7 or 20 months. Tyrosine hydroxylase positive cells in the substantia nigra pars compacta were quantified in a blinded manner. Neurochemical analysis of neurotransmitters and amino acids was performed in the striatum using high performance liquid chromatography.

Results

Mobility was increased by Mn, no significant difference due to the transgenes could be found. Striatal Mn content was significantly increased about threefold. Quantification of dopaminergic cells in the substantia nigra pars compacta showed a significant cell loss in aged mice (−10%) but no effect of Mn or transgenes (3-way ANOVA with factors gene, Mn and age). In 7 months old mice, neurochemical analysis showed interactions between transgene and Mn exposure for the ratio homovanillic acid : dopamine as well as aspartate (2-way ANOVA with factors gene and Mn). These values were increased in human α-syn-expressing compared to non-transgenic mice which were control-fed (17 and 11%, respectively). There was no increase when animals obtained Mn-enriched food. Contrary, mutated α-syn-expressing mice showed an increase compared to non-transgenic and human α-syn-expressing mice only when they obtained Mn-enriched food. Analysis of the same parameters in the 20 months old mice did not reveal any significant changes.

Conclusions

Under our experimental conditions, Mn and α-syn, wild-type and doubly mutated, did not induce signs of neurodegeneration, neither separately nor in interaction. However, Mn interferes with the dopamine system through human α-syn: manganese exposure decreased DA turnover in the striatum of mice expressing human α-syn wild-type. This effect was lost by the two parkinsonism inducing mutations.

Declarations

Acknowledgements

Supported by the Medical-Scientific fund of the Mayor of Vienna #2578 to C.P.

Authors’ Affiliations

(1)
Center for Brain Research, Medical University of Vienna
(2)
Core Unit for Medical Statistics and Informatics, Medical University of Vienna
(3)
Environmental and Occupational Health Science Institute

Copyright

© Pifl et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.

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