Volume 9 Supplement 2

15th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access

Role of sensory neurons on pancreatic beta cell function and on development of insulin resistance

  • Barna Peitl1Email author,
  • Róbert Döbrönte1,
  • László Drimba1,
  • Réka Sári1,
  • József Németh1 and
  • Zoltán Szilvássy1
BMC Pharmacology20099(Suppl 2):A54

https://doi.org/10.1186/1471-2210-9-S2-A54

Published: 12 November 2009

Background

To investigate the role of capsaicin-sensitive sensory afferent nerves on the pancreatic beta cell function and on the development of insulin resistance in genetically obese, insulin-resistant Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods

At the age of 6 weeks, OLETF rats were divided into two groups. The control group was treated with the vehicle for capsaicin, and the capsaicin group was treated with a single subcutaneous dose of 50 mg/kg capsaicin. The next 19 weeks, the metabolic variables (body weight gain, ingested food and water, stool and urine production) were measured by means of metabolic cages. At the end of the treatment period, the glucose-stimulated insulin response was determined by an oral glucose tolerance test (OGTT), whole body insulin sensitivity was determined by means of hyperinsulinaemic euglycaemic glucose clamping, and the hepatic glucose production (HGP) as well as insulin-stimulated peripheral glucose uptake (PGU) were determined by means of [3H]glucose infusion. Fasting plasma insulin levels were determined by RIA and fasting blood glucose values by the glucose oxidase method. Pancreatic beta cell function was characterized by the HOMA-B index based on fasting insulin and glucose levels.

Results

The body weight of the capsaicin-treated group was significantly lower than that of the control group. There were no changes in the other metabolic parameters. During the OGTT, the control group had a reduced glucose-stimulated response compared to the capsaicin-treated group and the area under the curve values were 1844 ± 124, and 1287 ± 87, respectively (p < 0.5). The whole body insulin sensitivity improved (from 9.4 ± 1.8 to 15.6 ± 2.1 mg/kg/min) significantly according to the improvement in HGP (from 7.5 ± 1.5 to 12.9 ± 3.1 mg/kg/min) and PGU (from 6.7 ± 1.2 to 2.8 ± 1.1 mg/kg/min). There was no difference in pancreatic beta cell function between the two treatment groups.

Conclusion

Capsaicin-sensitive sensory afferents play role in the development of obesity and insulin resistance in OLETF rat. To explore the interaction between the CCK1 and TRPV1 receptor in the vagal afferents, further experiments are needed.

Declarations

Acknowledgements

The work was supported by the Hungarian Scientific Research Fund (No. 74162).

Authors’ Affiliations

(1)
Department of Pharmacology and Pharmacotherapy, University of Debrecen

Copyright

© Peitl et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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