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Role of sensory neurons on pancreatic beta cell function and on development of insulin resistance

Background

To investigate the role of capsaicin-sensitive sensory afferent nerves on the pancreatic beta cell function and on the development of insulin resistance in genetically obese, insulin-resistant Otsuka Long-Evans Tokushima Fatty (OLETF) rats.

Methods

At the age of 6 weeks, OLETF rats were divided into two groups. The control group was treated with the vehicle for capsaicin, and the capsaicin group was treated with a single subcutaneous dose of 50 mg/kg capsaicin. The next 19 weeks, the metabolic variables (body weight gain, ingested food and water, stool and urine production) were measured by means of metabolic cages. At the end of the treatment period, the glucose-stimulated insulin response was determined by an oral glucose tolerance test (OGTT), whole body insulin sensitivity was determined by means of hyperinsulinaemic euglycaemic glucose clamping, and the hepatic glucose production (HGP) as well as insulin-stimulated peripheral glucose uptake (PGU) were determined by means of [3H]glucose infusion. Fasting plasma insulin levels were determined by RIA and fasting blood glucose values by the glucose oxidase method. Pancreatic beta cell function was characterized by the HOMA-B index based on fasting insulin and glucose levels.

Results

The body weight of the capsaicin-treated group was significantly lower than that of the control group. There were no changes in the other metabolic parameters. During the OGTT, the control group had a reduced glucose-stimulated response compared to the capsaicin-treated group and the area under the curve values were 1844 ± 124, and 1287 ± 87, respectively (p < 0.5). The whole body insulin sensitivity improved (from 9.4 ± 1.8 to 15.6 ± 2.1 mg/kg/min) significantly according to the improvement in HGP (from 7.5 ± 1.5 to 12.9 ± 3.1 mg/kg/min) and PGU (from 6.7 ± 1.2 to 2.8 ± 1.1 mg/kg/min). There was no difference in pancreatic beta cell function between the two treatment groups.

Conclusion

Capsaicin-sensitive sensory afferents play role in the development of obesity and insulin resistance in OLETF rat. To explore the interaction between the CCK1 and TRPV1 receptor in the vagal afferents, further experiments are needed.

Acknowledgements

The work was supported by the Hungarian Scientific Research Fund (No. 74162).

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Correspondence to Barna Peitl.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Peitl, B., Döbrönte, R., Drimba, L. et al. Role of sensory neurons on pancreatic beta cell function and on development of insulin resistance. BMC Pharmacol 9, A54 (2009). https://doi.org/10.1186/1471-2210-9-S2-A54

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Keywords

  • Insulin Resistance
  • Capsaicin
  • Oral Glucose Tolerance Test
  • Hepatic Glucose Production
  • Fast Plasma Insulin