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Volume 9 Supplement 2

15th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

A single neurotoxic dose of MDMA decreases BDNF expression in the frontoparietal cortex but not in the hippocampus

BMC Pharmacology volume 9, Article number: A41 (2009) Cite this article

Background

The popular recreational abuse drug 3,4-methylenedioxymethamphetamine (MDMA, 'Ecstasy') produces acute and long-lasting deficits in several markers of the serotonergic (5-HT) system. BDNF (brain derived neurotrophic factor) is a prominent trophic factor of serotonergic fibers. The aim of this study was to characterize the damage of serotonergic fibers in the frontoparietal cortex and hippocampus in parallel with the expression of BDNF.

Methods

Male Dark Agouti rats (7 weeks old) were treated with a single (15 mg/kg, i.p.) dose of MDMA. Density of tryptophan hydroxylase (TpH) immunoreactive (ir) fibers was quantified 3 days and 3 weeks after treatment. BDNF protein and mRNA levels were measured by ELISA and quantitative PCR method 24 hours and 3 weeks after treatment.

Results

Findings from TpH-ir fiber density revealed significant (30-50%) decrease, compared to baseline, both 3 days and 3 weeks after treatment in both hippocampus and the frontoparietal cortex. Results from ELISA showed that BDNF level in the frontoparietal cortex dropped significantly (30%) 24 hours and 3 weeks after treatment. This decrease in BDNF concentration was also confirmed with the quantitative PCR method. In contrast, no such a reduction was found in the hippocampus.

Conclusion

Our findings show that administration of a single dose of MDMA, a neurotoxic agent, leads to a significant drop in the TpH-ir axon density with a parallel decrease in BDNF levels 3 days and 3 weeks after treatment in the frontoparietal cortex. In contrast, in the hippocampus, the reduction of TpH-ir axon density is not accompanied by the decrease of BDNF level. These results highlight regional differences in the expression of BDNF, the main neurotrophic factor of serotonergic fibers, in the terminal serotonergic areas after a neurotoxic dose of MDMA.

Acknowledgements

Supported by the EC, LSHM-CT-2004-503474.

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Authors and Affiliations

  1. Institute of Pharmacodynamics, Semmelweis University, 1089, Budapest, Hungary

    Szilvia Vas, Diána Kostyalik, Csaba Ádori & György Bagdy

  2. Institute of Experimental Medicine, Hungarian Academy of Sciences, 1450, Budapest, Hungary

    Rómeó D Andó

Authors
  1. Szilvia Vas
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  2. Diána Kostyalik
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  3. Csaba Ádori
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  4. Rómeó D Andó
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  5. György Bagdy
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Corresponding author

Correspondence to György Bagdy.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( https://creativecommons.org/licenses/by/2.0 ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cite this article

Vas, S., Kostyalik, D., Ádori, C. et al. A single neurotoxic dose of MDMA decreases BDNF expression in the frontoparietal cortex but not in the hippocampus. BMC Pharmacol 9 (Suppl 2), A41 (2009). https://doi.org/10.1186/1471-2210-9-S2-A41

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  • DOI: https://doi.org/10.1186/1471-2210-9-S2-A41

Keywords

  • Neurotrophic Factor
  • Trophic Factor
  • BDNF Level
  • Abuse Drug
  • Fiber Density

BMC Pharmacology

ISSN: 1471-2210