Volume 9 Supplement 2

15th Scientific Symposium of the Austrian Pharmacological Society (APHAR)

Open Access

Characterization of receptors involved in serotonin contractions of isolated human umbilical artery in uncomplicated pregnancy

  • Miroslav Radenković1Email author,
  • Nebojša Radunović2,
  • Paja Momčilov2 and
  • Leposava Grbović1
BMC Pharmacology20099(Suppl 2):A14

https://doi.org/10.1186/1471-2210-9-S2-A14

Published: 12 November 2009

Background

Serotonin (5-HT), is a vasoactive substance involved in physiological functioning of fetoplacental blood flow and continuous modulation of smooth muscle tone in umbilical vein and arteries. Since umbilical blood vessels have been shown to be deficient in autonomic innervation, the action of local autocrine vasoactive substances (including serotonin) is of considerable importance. Although seven main groups of 5-HT receptors (5-HT1-7) have been characterized, it has been established that only several 5-HT receptor subtypes may be involved in vascular effects of this biogenic amine. Therefore, the aim of this study was to characterize receptors involved in serotonin-induced effect on isolated human umbilical artery (HUA) in uncomplicated pregnancy.

Methods

The experiments were performed on intact vascular rings of HUA isolated from umbilical cords that were obtained immediately after vaginal delivery in women with uncomplicated pregnancy. Only the remnant tissue, which would have been otherwise disposed of, has been utilized. Isometric tension of suspended artery rings was continuously recorded. Contraction induced by each concentration of 5-HT was later expressed as a percentage of the maximal contraction induced by Krebs bicarbonate solution with 60 mM KCl.

Results

5-HT (1 nM - 30 μM) produced concentration-dependent contractions of HUA. Control contractions produced by 5-HT were notably reduced by methiothepin (a nonselective 5-HT1/5-HT2 receptor antagonist; 0.01-1 μM), with typical irreversible competitive antagonism exposed. On the other hand, increasing concentrations of ketanserin (a selective 5-HT2A receptor antagonist; 0.03-0.3 μM) significantly shifted 5-HT control curves to the right in a concentration-dependent manner with reversible competitive antagonism shown.

Conclusion

Schild's analysis of the effect produced by ketanserin, taken together with the results obtained with methiotepin, suggest that the transduction mechanism of HUA responses to serotonin in uncomplicated pregnancy involves activation of a mixed population of 5-HT1 and 5-HT2A receptors.

Declarations

Acknowledgements

This research was supported by grant 145015B from the Ministry of Science, Serbia.

Authors’ Affiliations

(1)
Department of Pharmacology, Clinical Pharmacology and Toxicology, Medical Faculty, University of Belgrade
(2)
Institute of Obstetrics and Gynecology, Medical Faculty, University of Belgrade

Copyright

© Radenković et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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