Skip to main content

Table 6 Effects of rapacuronium on the rate of [3H]ACh association and dissociation

From: Divergence of allosteric effects of rapacuronium on binding and function of muscarinic receptors

 

40 nM [3H]ACh

+ 10 μM rapacuronium

 

kobs [min-1]

koff [min-1]

koff [min-1]

M1

2.74 ± 0.25

0.294 ± 0.015

0.917 ± 0.046*

M2

1.58 ± 0.14

0.507 ± 0.025

0.245 ± 0.012*

M3

1.43 ± 0.13

0.226 ± 0.011

0.923 ± 0.046*

M4

2.01 ± 0.18

0.373 ± 0.019

0.355 ± 0.018

M5

3.50 ± 0.31

0.112 ± 0.006

0.378 ± 0.019*

  1. Observed rates of association (kobs) of 40 nM [3H]ACh with and dissociation (koff) from individual subtypes of muscarinic receptors were obtained by fitting Eq. 6 to data in Figure 8 and Eq. 7c to data in Figure 9. Values are means ± SE of fits to 3 independent experiments performed in quadruplicates. *P < 0.05; significantly different from control ([3H]ACh alone) by t-test.
Back to article page

BMC Pharmacology

ISSN: 1471-2210