The peripheral antinociceptive effect of DAMGO and 6β-glycine-substituted 14-O-methyloxymorphone (HS-731) after systemic administration in a mouse visceral pain model
© Al-Khrasani et al; licensee BioMed Central Ltd. 2007
Published: 14 November 2007
Inhibition of nociception through activation of peripheral μ opioid receptors (MOR) avoiding the central adverse effects of opioids has added a new possibility to manage pain control. Here we report the antinociceptive effects of the peripherally restricted MOR agonist HS-731, the peptide DAMGO, and morphine in the writhing test in mice. S.c. and i.c.v. HS-731 dose-dependently and completely inhibited writhing, being 24–598 times more potent than the two MOR-selective agonists DAMGO and morphine. However, extremely high s.c./i.c.v. potency ratios were calculated for HS-731 and DAMGO and much lower for morphine. Remarkably, a long duration of action was induced by HS-731 and much shorter by morphine and DAMGO. The antinociceptive effects of systemic opioids were reversed by s.c. naloxone while i.c.v. administration of the MOR selective antagonist CTAP significantly abolished the antinociceptive effect of s.c. morphine but completely failed to antagonize the effects of systemic HS-731 or DAMGO. In addition, in the rat vas deferens HS-731 and DAMGO, but not morphine, showed high intrinsic efficacy and naltrexone-sensitive agonist response at MOR by depressing electrically-evoked contractions of this organ. These data demonstrate that the selectivity and high efficacy of HS-731 and DAMGO at peripheral MOR as well as their inability to cross the blood brain barrier are a cornerstone for producing peripheral antinociception after systemic administration.
This work was supported by grants from the European Committee (QLK6-0999; EPILA), Hungarian research grants (OTKA K-60999 and ETT-441/2006) and the Austrian Science Foundation (P15481).
This article is published under license to BioMed Central Ltd.