Skip to content


Open Access

Analysis of smooth muscle function in IRAG knockout mice

  • Matthias M Desch1Email author,
  • Katja Sigl2,
  • Beate Spiessberger2,
  • Dominik Bernhard2,
  • Angela Jochim2,
  • Jörg W Wegener2,
  • Franz Hofmann2 and
  • Jens Schlossmann1, 2
BMC Pharmacology20077(Suppl 1):P14

Published: 25 July 2007


Protein KinaseSmooth MuscleVascular ToneImportant SignalingGastrointestinal Motility

Signaling by NO/cGMP relaxes various smooth muscles, modulating thereby vascular tone and gastrointestinal motility. An important signaling pathway of cGMP-dependent protein kinase type I (cGKI)-dependent relaxation is mediated by IRAG (IP3 Receptor Associated cGKI substrate) which is highly expressed in smooth muscle tissues. In order to elucidate the physiological role of IRAG we generated IRAG knockout mice by targeted deletion of exon 3.

The cGMP dependent relaxation of hormone induced contraction on gastrointestinal tissues of colon and jejunum as well as aortic vessels is significantly affected in IRAG knockout mice in comparison to wild type animals. The analysis of smooth muscle function indicates that IRAG is essentiaI for NO/cGMP/cGKI signaling, especially for regulating muscular tone.

Authors’ Affiliations

Institut für Pharmakologie und Toxikologie, Universität Regensburg, Germany
Institut für Pharmakologie und Toxikologie, TU München, Germany


© Desch et al; licensee BioMed Central Ltd. 2007

This article is published under license to BioMed Central Ltd.