Table 3 Hemolysis of 6.5 × 10⁷(2.6 x10⁷ /ml) SRBC by celecoxib (80 μM) and tamoxifen (20 and 40 μM). The SRBC (2.5 ml) were incubated in a shaking bath for 19 h at 37°C. The cells were centrifuged and the supernates analyzed for oxyhaemoglobin by absorption at 413 nm. Complete hemolysis was calculated from the absorption of the H₂O lysate of the centrifuged cells of the controls. Controls for absorption at 413 nm of the reagents in the PBS/BSA vehicle as well as controls that contained known amounts of SRBC lysate were used to measure the effects of NSAIDs, if any, on oxyhemoglobin absorption.

From: Cyclooxygenase expression is not required for release of arachidonic acid from cells by some nonsteroidal anti-inflammatory drugs and cancer preventive agents