Table 1 Release of AA from HCT-15 cells by celecoxib (50 μM) and NS-398 (52 μM). HCT-15 cells, 0.3 to 1.0 × 105/dish, were incubated with the celecoxib, NS-398 or control for 19 h at 37°C. After centrifugation, culture fluids (200 μl) were counted for radioactivity.
Drug tested | cpm/200 μl | AA Release % |
|---|---|---|
None | 706 | 2.7 |
| Â | 947 | 3.6 |
(Control MEM/BSA) | 943 | 3.6 |
| Â | 799 | 3.1 |
| Â | 793 | 3.0 |
|  | m = 838 ± 46.8 | m = 3.2 ± 0.18 |
Celecoxib (50 μM) | 3740 | 14.4 |
| Â | 4441 | 17.1 |
| Â | 3851 | 14.8 |
| Â | 4166 | 16.0 |
| Â | 4413 | 16.9 |
|  | m = 4122 ± 143 | m = 15.8 ± 0.54 |
NS398 (52 μM) | 1140 | 4.4 |
| Â | 1328 | 5.1 |
| Â | 1223 | 4.7 |
| Â | 1249 | 4.8 |
| Â | 1186 | 4.6 |
|  | m = 1225 ± 32 | m = 4.7 ± 0.12 |