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Open Access

Endothelium-mediated actions of the ANP/cGMP system

  • Markus N Kruse1,
  • Karim Sabrane1,
  • Larissa Fabritz2,
  • Bernd Zetsche1,
  • Danuta Mitko1,
  • Boris V Skryabin3,
  • Melanie Zwiener2,
  • Hideo A Baba4,
  • Masashi Yanagisawa5 and
  • Michaela Kuhn1, 6Email author
BMC Pharmacology20055(Suppl 1):S31

https://doi.org/10.1186/1471-2210-5-S1-S31

Published: 16 June 2005

Atrial natriuretic peptide (ANP) via its vasodilating and diuretic effects has an important physiological role in the maintenance of arterial blood pressure and volume. Its guanylyl cyclase-A (GC-A) receptor is highly expressed in vascular endothelium, but the functional relevance is controversial. To dissect the endothelium – mediated actions of ANP in vivo, we inactivated the GC-A gene selectively in endothelial cells by homologous loxP/Tie2-Cre-mediated recombination. Notably, despite full preservation of the direct vasodilating effects of ANP, mice with endothelial-restricted GC-A deletion (EC GC-A KO mice) exhibited significant arterial hypertension and cardiac hypertrophy. Echocardiographic and Doppler flow evaluations demonstrated that cardiac output and stroke volume as well as the mean pressure gradient and maximal flow velocity across the aortic valve were increased in EC GC-A KO as compared to control mice with normal GC-A expression levels. Application of the Evan's Blue dilution technique showed that the total plasma volume of EC GC-A KO mice was increased by 11–13%, even under conditions of normal dietary salt intake. Infusion of ANP caused immediate raises in hematocrit in control but not in EC GC-A KO mice, indicating that ablation of endothelial GC-A completely prevented the acute contraction of intravascular volume by ANP. Furthermore, intravenous ANP acutely enhanced the rate of clearance of radio-iodinated albumin from the circulatory system in control mice but not in EC GC-A KO littermates. We conclude that GC-A – mediated increases in endothelial permeability are critically involved in the hypovolemic, hypotensive actions of ANP.

Authors’ Affiliations

(1)
Institute of Pharmacology and Toxicology, Universitätsklinikum Münster
(2)
Department of Cardiology and Angiology, Universitätsklinikum Münster
(3)
Institute of Experimental Pathology, Universitätsklinikum Münster
(4)
Institute of Pathology, Universität Ruhr-Essen
(5)
Department of Molecular Genetics and Howard Hughes, Medical Institute, Southwestern Medical Center
(6)
Institute of Physiology, University of Würzburg

Copyright

© BioMed Central Ltd 2005

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