Volume 5 Supplement 1

2nd International Conference of cGMP Generators, Effectors and Therapeutic Implications

Open Access

ATP-independent activation of natriuretic peptide receptors

  • Laura K Antos1,
  • Sarah E Abbey-Hosch1,
  • Darcy R Flora1 and
  • Lincoln R Potter1Email author
BMC Pharmacology20055(Suppl 1):P3

https://doi.org/10.1186/1471-2210-5-S1-P3

Published: 16 June 2005

Natriuretic peptide receptor A (NPR-A) is an essential cardiovascular regulator that is stimulated by atrial natriuretic peptide and brain natriuretic peptide, whereas natriuretic peptide receptor B (NPR-B) stimulates long bone growth in a C-type natriuretic peptide-dependent manner. Many reports indicate that ATP is essential for NPR-A and NPR-B activation. Current models suggest that natriuretic peptide binding to receptor extracellular domains causes ATP binding to intracellular kinase homology domains, which derepresses adjacent catalytic domains. Here, we report 100-fold activation of natriuretic peptide receptors in the absence of ATP. Addition of a nonhydrolyzable ATP analog had no effect at early time periods (seconds) but increased cGMP production about two-fold after longer incubations (minutes), consistent with a stabilization, not activation, mechanism. These data indicate that ATP does not activate natriuretic peptide receptors. Instead, ATP increases activity primarily by maintaining proper receptor phosphorylation status, but also serves a previously unappreciated enzyme stabilizing function.

Authors’ Affiliations

(1)
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota

Copyright

© BioMed Central Ltd 2005

Advertisement