Figure 4

Analysis of other known mechanisms of resistance to gemcitabine. (A) Quantitative real time RT-PCR analysis of factors implicated in the activation or degradation of gemcitabine in RL-7 and RL-G cells; 18S ribosomal RNA (18S), deoxycytidine kinase (dCK), high Km 5'-nucleotidase (cNII), 5'-3'-nucleotidase (dNT1) and mitochondrial 5'-nucleotidase (dNT2) (B) Quantitative real time PCR analysis of factors implicated in the transport and intracellular targeting of gemcitabine; human equilibrative nucleoside transporter 1 and 2 (ENT1; ENT2), ribonucleotide reductase M1 and M2 subunits (M1, M2) and DNA polymerase α (Pol). RT-PCR of these genes was performed as described in Material and Methods. Results are expressed as % of PCR arbitrary unit expression in RL-G cells related to RL-7 PCR arbitrary unit expression.