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Figure 7 | BMC Pharmacology

Figure 7

From: 15-deoxy-delta 12, 14-Prostaglandin J2 prevents reactive oxygen species generation and mitochondrial membrane depolarization induced by oxidative stress

Figure 7

Effect of 15d-PGJ 2 on tBH-induced ROS generation. (A): ROS generation by tBH. Cells were treated with H2DCF-DA for 20 min, followed by tBH for 20 min, and then the "total" (solid squares) and "cell-associated" (open squares) ROS generation was determined. Results indicated that tBH caused a dose-dependent ROS generation. At 1000 μM tBH, the "total" ROS production was ~121 RU, which was ~3× of basal levels (~40 FU). (B): 15d-PGJ2 reduced tBH-induced ROS generation. Cells were pretreated with 1 μM 15d-PGJ2 overnight, then challenged with 1000 μM tBH (without 15d-PGJ2) for 20 min. Results indicated that 15d-PGJ2 caused a statistically significant decrease of ROS generation. *p < 0.001.

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BMC Pharmacology

ISSN: 1471-2210