Table 4 Transgene expression in different human cancer cell lines. These values are determined in the same experiments as depicted in figures 7 and 8. A: Infectibility of the cell lines as defined by the percentage of adenovirus DNA seeded that was detected in the cells 2 days after infection. B: the transcription efficiency is defined by the ratio of the mRNA values divided by the number of adenoviral copies in the cells. C: The translation efficiency is the ratio of the luciferase activity / luciferase mRNA. D: Luciferase activity in 10³ RLU / mg protein. The absolute values are shown in italic. The values relative to the LS174T, which was arbitrary set as reference are indicated in plain text.

1

72

82

13

15

12

14

34

38

0.9

1

10

55

104

7

13

10

18

21

40

0.5

1

100

78

101

10

13

9

12

22

29

0.8

1

Average

96

14

15

36

1

SD

12

1

3

6

B

Transcription efficiency (mRNA / Ad DNA

MOI

Cell lines

U251

NCI H460

HepG2

A549

LS 174T

1

2.1

0.7

2.1

0.8

2.0

0.7

0.4

0.14

2.8

1

10

2.0

0.7

2.7

0.9

1.9

0.7

0.4

0.16

2.8

1

100

1.9

0.7

2.7

1.0

2.3

0.9

0.6

0.22

2.6

1

Average

0.72

0.91

0.76

0.17

1

SD

0.02

0.14

0.11

0.04

C

Translation efficiency (Luciferase / mRNA)

MOI

Cell lines

U251

NCI H460

HepG2

A549

LS 174T

1

40

2.8

12

0.8

13

0.9

9

0.6

14

1

10

127

2.2

48

0.8

38

0.7

28

0.5

58

1

100

316

1.6

152

0.8

140

0.7

78

0.4

198

1

Average

2.2

0.8

0.8

0.5

1

SD

0.6

0.04

0.2

0.1

D

Luciferase activity

MOI

Cell lines

U251

NCI H460

HepG2

A549

LS 174T

1

60

172

3

9.2

3

9.5

1

3.4

0.3

1

10

1381

158

92

10.5

69

7.9

27

3.1

9

1

100

46350

116

4061

10.1

3055

7.6

1016

2.5

400

1

Average

149

9.9

8.3

3.0

1

SD

29

0.7

1.0

0.4