Figure 1
From: Selective alteration of gene expression in response to natural and synthetic retinoids.
Expression levels of retinoic acid receptors and of transcriptional intermediary factors in HeLa cells. HeLa cells mRNA was extracted and analyzed by RT-PCR using specific primers to detect (A) hRARα, hRARβ, hRARγ, hRXRα, hRXRβ and hRXRγ transcripts; to confirm the lack of expression of hRARβ, hRARγ and hRXRγ by nested PCR (B) to characterize expression levels of nuclear coactivators (C) and nuclear corepressors (D). E) Western blot analysis of HeLa whole cell extracts. 100 μg of proteins were resolved by 8% SDS-PAGE and blotted onto a nitrocellulose membrane. This membrane was probed with antibodies specific for each indicated receptors, coactivators or corepressor. The left panel shows a silver-stained gel on which 10 μg of cell extract has been separated. Molecular masses are indicated in kDa.