A. Western blot analyses for identification of the 5-HT1Breceptor in homogenates of SMA from sham (n = 6) and DOCA-salt (n = 6) rats. Arrows = band or region of interest. B. Immunohistochemical staining of the 5-HT1B receptor in SMA sections from sham and DOCA-salt rats. Images are sections of SMA incubated with and without primary antibody and taken with 20X objective. M = media, A = adventitia, S = staining in rat brain, L = lumen (n = 6). C. Cumulative concentration response curve to CP 93129 vs. vehicle (H2O) in SMA contracted with U46619 in sham and DOCA-salt SMA. Data points represent mean ± SEM for the number of animals in parentheses.