Figure 1
From: cGMP and cAMP differentially regulate differentiation and function of brown adipocytes
Effects of chronic cAMP and cGMP treatment on brown fat cell differentiation. A The protocol for adipogenic differentiation of BAT-derived preadipocytes and the media (DM, differentiation medium; IM, induction medium; Dexa, dexamethasone; IBMX, 3-isobutyl-methyl-xanthine; T3, triiodothyronine) used at the different time points (upper panel). Effects of chronic 8-Br-cAMP (cAMP; 200 µM) and 8-pCPT-cGMP (cGMP; 200 µM) treatment during differentiation of brown preadipocytes on lipid accumulation as assessed by Oil RedO staining (day 7) (lower panel). B TG content of differentiated brown adipocytes (day 7) after chronic treatment with cAMP or cGMP during differentiation. Data are represented as means ± SEM; * p < 0.05.