Figure 3
SalB inhibits MMP-9 activity specifically and competitively. (A) Kinetic analysis of SalB against MMP-9 CD through double reciprocal plots of 1/V versus 1/[thiopeptolide]. (B) Secondary plot of Kmapp versus different SalB concentration. The Ki of SalB against MMP-9 CD is calculated to be 79.2 μM. (C) Representative zymography results for SalB inhibition on native MMP-2, MMP-9 activity in vitro. (D) Quantitative data from Fig. 3C for SalB inhibition on MMP-9, MMP-2 activity. (n = 3 independent experiments).