Volume 7 Supplement 1

3rd International Conference on cGMP Generators, Effectors and Therapeutic Implications

Open Access

Signaling of NO/cGMP via IRAG

BMC Pharmacology20077(Suppl 1):S49

DOI: 10.1186/1471-2210-7-S1-S49

Published: 25 July 2007

Signaling by NO/cGMP/cGMP-dependent kinase I (cGKI) is important for a variety of physiological functions comprising relaxation of smooth muscle and inhibition of platelet aggregation. An important pathway of this signaling cascade includes the inositol 1,4,5-trisphosphate receptor I (IP3RI) associated protein cGMP kinase substrate (IRAG). This protein interacts in a trimeric macrocomplex with cGKIβ and the IP3RI. To get insight into the physiological function of IRAG two different mice strains were generated by targeted deletion: (1) IRAGΔ12/Δ12 with an exon 12 deletion disrupting the IRAG/IP3RI interaction. (2) IRAG-/- with an exon 3 deletion generating an IRAG knockout mutant.

Analysis of IRAGΔ12/Δ12 platelet aggregation in vitro using collagen and thrombin as agonists and Fura2 calcium measurements revealed that IP3RI/IRAG interaction is essential for NO/cGMP signaling mediating inhibition of platelet aggregation. Furthermore, it was shown that IP3RI/IRAG interaction is essential for the NO-dependent prevention of thrombus formation.

Relaxation of hormone-contracted aortic and longitudinal colonic smooth muscle by cGMP was abolished in IRAGΔ12/Δ12 mice and IRAG knockout mice indicating an essential role of IRAG for NO/cGMP-dependent smooth muscle relaxation. The vascular function of IRAG was underlined by a lack of NO-dependent blood pressure reduction in IRAGΔ12/Δ12 mice. These studies suggest that cGKI/IRAG/IP3RI is an essential signaling pathway modulating cardiovascular functions.

Authors’ Affiliations

(1)
Institut für Pharmakologie und Toxikologie, Universität Regensburg

Copyright

© Schlossmann; licensee BioMed Central Ltd. 2007

This article is published under license to BioMed Central Ltd.

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