A study comparing the actions of gabapentin and pregabalin on the electrophysiological properties of cultured DRG neurones from neonatal rats

Background Gabapentin and pregabalin have wide-ranging therapeutic actions, and are structurally related to the inhibitory neurotransmitter GABA. Gabapentin, pregablin and GABA can all modulate voltage-activated Ca2+ channels. In this study we have used whole cell patch clamp recording and fura-2 Ca2+ imaging to characterise the actions of pregabalin on the electrophysiological properties of cultured dorsal root ganglion (DRG) neurones from neonatal rats. The aims of this study were to determine whether pregabalin and gabapentin had additive inhibitory effects on high voltage-activated Ca2+ channels, evaluate whether the actions of pregabalin were dependent on GABA receptors and characterise the actions of pregabalin on voltage-activated potassium currents. Results Pregabalin (25 nM – 2.5 μM) inhibited 20–30% of the high voltage-activated Ca2+ current in cultured DRG neurones. The residual Ca2+ current recorded in the presence of pregabalin was sensitive to the L-type Ca2+ channel modulator, Bay K8644. Saturating concentrations of gabapentin failed to have additive effects when applied with pregabalin, indicating that these two compounds act on the same type(s) of voltage-activated Ca2+ channels but the majority of Ca2+ current was resistant to both drugs. The continual application of GABA, the GABAB receptor antagonist CGP52432, or intracellular photorelease of GTP-γ-S had no effect on pregabalin-induced inhibition of Ca2+ currents. Although clear inhibition of Ca2+ influx was produced by pregabalin in a population of small neurones, a significant population of larger neurones showed enhanced Ca2+ influx in response to pregabalin. The enhanced Ca2+ influx evoked by pregabalin was mimicked by partial block of K+ conductances with tetraethylammonium. Pregabalin produced biphasic effects on voltage-activated K+ currents, the inhibitory effect of pregabalin was prevented with apamin. The delayed enhancement of K+ currents was attenuated by pertussis toxin and by intracellular application of a (Rp)-analogue of cAMP. Conclusions Pregabalin reduces excitatory properties of cultured DRG neurones by modulating voltage-activated Ca2+ and K+ channels. The pharmacological activity of pregabalin is similar but not identical to that of gabapentin. The actions of pregabalin may involve both extracellular and intracellular drug target sites and modulation of a variety of neuronal conductances, by direct interactions, and through intracellular signalling involving protein kinase A.


Background
Gabapentin (Neurotonin ® ) and pregabalin (S(+)-3-isobutyl GABA) were both originally designed as GABA mimetics ( Figure 1A), with the intention that they would be able to cross the blood-brain barrier and interact with GABAergic systems and enhance GABA mediated inhibition. Although gabapentin appears to have diverse therapeutic utility in the treatment of pain disorders [1], psychiatric illnesses [2] and epilepsy [3], there is controversy regarding its molecular mechanisms of action. Whether the actions of gabapentin and pregabalin are mediated through GABAergic mechanisms or GABA receptors remains particularly contentious [4][5][6]. However, high affinity binding sites for gabapentin and pregabalin on distinct α 2 δ subunits of voltage-activated calcium channels have been identified and characterised [7]. For this reason voltage-activated Ca 2+ channels remain primary candidate sites of action for these novel anticonvulsant and antihyperalgesic drugs. Functional data from studies on gabapentin and pregabalin also support this contention. Specifically, both gabapentin and pregabalin inhibited hyperalgesia [8,9], attenuated evoked Ca 2+ influx into brain slices and reduced evoked transmitter release [10]. Additionally, gabapentin and pregabalin inhibited multiple firing of action potentials evoked by 300 ms depolarising current commands in cultured sensory DRG neurones [5].
Our previous studies have focused on the inhibitory effects of gabapentin (0.25-25 µM) on whole cell voltageactivated Ca 2+ currents and K + stimulated Ca 2+ entry measured with fura-2. The cellular model systems used were primary cultures of dorsal root ganglion (DRG) neurones from 1-4 day old rats and differentiated F-11 cells (embryonic rat DRG × neuroblastoma hybrid cell line) [11,12].
In this present study the effects of pregabalin and gabapentin on the electrophysiological properties of cultured neonatal rat DRG neurones were measured with particular reference to Ca 2+ entry through high voltage-activated channels and enhancement of K + conductances. We had two specific aims for this project. The first was to determine whether gabapentin and pregabalin have the same mechanisms of action, by examining whether saturating concentrations of gabapentin and pregabalin act in an additive manner to attenuate Ca 2+ influx. The second aim was to further evaluate GABA receptors as target sites for these drugs in sensory neurones. This latter element to the study was conducted because it has been proposed that specific GABA B receptors with a gb1a-gb2 heterodimer composition are the sites of agonist activity of gabapentin. These specific receptors can be coupled to an inwardly rectifying K + channel and / or voltage-activated Ca 2+ channels to dampen neuronal electrical excitability [13][14][15]. How-ever, in certain situations gabapentin and the GABA B receptor agonist, baclofen, have different actions. Weaver mutant mice (wv/wv) are insensitive to both gabapentin and baclofen, however in control littermates, Weaver control mice (+/+, wv/+), only baclofen evoked a K + current [16].

Actions of pregabalin on voltage-activated calcium currents
The firing of multiple action potentials in response to a sustained depolarising current command is a property of a sub-population (under 20 %) of cultured DRG neurones. Application for 3 minutes, of either pregabalin (PGB; 2.5 µM) or gabapentin (GBP; 2.5 µM) reduced the frequency of action potential spikes evoked by 300 ms depolarising current step commands applied every 30 s [5]. Gabapentin attenuated repetitive action potential firing as measured by a reduction in the mean number of evoked action potentials during 300 ms depolarisations from 7 to 2 (n = 3, p < 0.01). Pregabalin (2.5 µM) reversibly reduced the number of action potentials during 300 ms depolarisations from 8 to 3 (n = 5, p < 0.01). However, pregabalin and gabapentin did not significantly alter the properties (amplitude, duration & threshold) of single action potentials evoked by 5 ms depolarising current commands.
Consistent with our previous work [11,12], whole cell voltage-activated Ca 2+ currents (I Ca ) recorded from DRG neurones were reversibly attenuated (22 ± 11%; n = 7) by 2.5 µM gabapentin. Similarly, 3 minutes application of pregabalin (25 nM -2.5 µM; Figure 1B &1C; table 1) inhibited the mean Ca 2+ current amplitude, measured at the peak of the inward current, and at the end of a 100 ms voltage step command to 0 mV. The inhibitory actions of pregabalin were not accompanied by any shift in the voltage-dependence of activation for I Ca or by any change in holding or leak currents. At least partial recovery of I Ca was observed 5 minutes after removal of the pregabalin-containing perfusion pipette ( Figure 1B &1C). A very low concentration of pregabalin (0.25 nM) failed to produce significant inhibition of I Ca . However, no clear dosedependent relationship was established for the inhibitory actions of pregabalin, and a major part of the voltage-activated Ca 2+ current was insensitive to the drug. It was also apparent that there was considerable variability in the sensitivity of the DRG neurones to any given dose of pregabalin. Some neurones did not respond to 2.5 µM pregabalin while in a few neurones this same concentration produced 60 % inhibition of I Ca ( Figure 1D).
Distinct voltage-activated Ca 2+ channels, defined by their alpha 1 subunit, have been suggested to be selective target sites for gabapentin and related drugs like pregabalin. Figure 1 Pregabalin inhibits Ca 2+ currents. A) Structure of GABA (γ-aminobutyric acid), gabapentin (1-(aminoethyl)cyclohexane acetic acid) and pregabalin (S(+)-3-isobutyl GABA). B & C) Traces of high voltage-activated Ca 2+ currents evoked from a holding potential of -90 mV by a depolarising step command to 0 mV showing inhibition by pregabalin (2.5 µM). B) Shows that pressure ejection of choline chloride extracellular solution does not induce inhibition of the Ca 2+ current but in the same neurone pregabalin does produce a response. Traces show a control Ca 2+ current (Ctrl), the current unaffected by application of choline chloride recording solution (ChCl), inhibition of current by 3 minutes application of pregabalin (PGB) and the current at 5 minutes recovery (Rec). C) Traces show a control Ca 2+ current (Ctrl), the inhibition of current after 3 minutes application of pregabalin (PGB) and full recovery of the current 5 minutes after removal of the drug pipette (Rec). D) Graph showing the distribution of inhibitory responses produced by 0.025 -2.5 µM pregabalin. Each symbol represents a result from a different experiment. With this in mind, the 1,4-dihydropyridine L-type Ca 2+ channel agonist Bay K8644 was used to determine whether pregabalin was inhibiting L-type channels. Previously, we found that in the continual presence of gabapentin, Bay K8644 enhanced I Ca [12]. In this present study similar results were obtained. After inhibiting part of the current with pregabalin (2.5 µM), Bay K8644 (1 µM) was applied with pregabalin and the enhanced I Ca was measured at its peak and at the end of a 100 ms voltage step command to 0 mV ( Figure 2). The percentage increases in current seen with Bay K8644 in the presence of either gabapentin or pregabalin were slightly less than those seen under control conditions in cultured DRG neurones [17]. This is consistent with some L-type Ca 2+ current modulation by pregabalin and gabapentin in DRG neurones. Taken together these data suggest that neither gabapentin nor pregabalin are selectively inhibiting Ltype I Ca in DRG neurones. This contrasts with the effects of gabapentin on cortical pyramidal neurones where inhibition of L-type Ca 2+ channels appears to be the predominant mechanism of action [18].

Actions of pregabalin on calcium influx through voltageactivated channels, measured using fura-2 imaging
Given the variable and rather modest but reversible inhibitory actions of pregabalin on I Ca we tested the actions of pregabalin on K + -evoked Ca 2+ influx using fura-2 imaging. An extracellular solution containing 30 mM K + was used to depolarise the DRG neurones and activate three consistent Ca 2+ transients [11,12]. Pregabalin (2.5 µM) was applied during the second K + -evoked depolarisation and it produced a mixture of reversible effects on the Ca 2+ transients. In 4 of 24 neurones the Ca 2+ flux was decreased by 54 ± 13 % (p<0.05) but in 20 neurones from the same cultures pregabalin evoked a mean increase in Ca 2+ flux to 185 ± 20 % (p<0.05) of the control. Raising the pregabalin concentration to 25 µM increased the proportion of inhibitory responses (n = 21 out of 49 neurones) but enhancement of Ca 2+ transients was still observed in the remaining 28 neurones (Figure 3). However, 250 µM pregabalin caused an increase in K + -evoked Ca 2+ influx in all neurones studied (n = 31). Although in some cells good recovery from pregabalin actions was observed (figure 3B) the inhibitory effect was often not associated with recovery. This may reflect long lasting effects of pregabalin. Run-down in some cells can not be completely ruled out but under control conditions the mean level of rundown of three K + -evoked Ca 2+ transients was only 8.4 ± 1.5 % (n = 39). Similarly, the mean control level of increase in K + -evoked Ca 2+ transients was only 7.2 ± 1.3 % (n = 35).
To investigate the different responses to pregabalin, the sizes of cell somas were measured and compared. Although there is overlap, figure 4 shows that enhancement of K + -evoked Ca 2+ transients by pregabalin was mainly seen in neurones with larger cell somas and that in smaller neurones pregabalin produced inhibitory effects.  Figure 5A,5B). The second approach was to explore a possible role of Na + / Ca 2+ exchange by bathing cells in choline chloride-based medium containing only 1 mM Na + . Under these conditions the contribution of the Na + / Ca 2+ exchanger to handling of intracellular Ca 2+ loads will be minimal. In choline chloride-based medium pregabalin produced both enhancement and inhibition of total Ca 2+ flux in 7 and 3 neurones respectively. Enhancement in Ca 2+ flux by pregabalin under these experimental ***P < 0.001; **P < 0.01 and *P < 0.05. Each value is given ± S.E.M. Data shows inhibition but a lack of dose-dependent actions on I Ca of a wide range of pregabalin concentrations. Although varied responses were observed it was clear that a major component of I Ca was resistant to inhibition by pregabalin.
Bay K8644 enhanced pregabalin-insensitive current suggesting that L-type Ca 2+ channels are still available for modulation by the 1,4-dihydropyridine agonist   conditions, suggest that inhibition of Na + / Ca 2+ exchange is not the main mechanism by which pregabalin enhances K + -evoked Ca 2+ flux ( Figure 5C). However, detailed analysis was made difficult by poor recovery of even the first Ca 2+ transient evoked in low extracellular Na + which does suggest that Na + / Ca 2+ exchange is an important homeostatic mechanism in DRG neurones. In conclusion these results indicate that modulation of Ca 2+ -induced Ca 2+ Different responses to pregabalin were observed in different populations of cultured DRG neurones   Inhibitory modulation of potassium conductances could result in increased K + -evoked Ca 2+ transients. To test this alternative mechanism, a relatively low concentration, 5 mM, of tetraethylammonium (TEA) was applied with NaCl-based extracellular medium and DRG neurones were stimulated with 30 mM KCl. Attenuation of potassium conductances by TEA markedly increased the K +evoked Ca 2+ flux in all eight DRG neurones studied, mimicking in part the action of pregabalin ( Figure 5D).

Do pregabalin and gabapentin have additive effects on cultured dorsal root ganglion neurones?
Pregabalin and gabapentin have related chemical structures and appear to have similar but usually modest inhibitory effects on Ca 2+ currents. Both pregabalin and gabapentin at a concentration of 2.5 µM produced a maximum level of current inhibition. Simultaneous application of pregabalin (2.5 µM) and gabapentin (2.5 µM) produced modest but significant inhibition of I Ca at the peak of the current and at the end of the stimulus ( Figure  6A). However, the percentage inhibition of I Ca produced was not significantly different for 2.5 µM gabapentin alone (22 ± 11%; n = 7), 2.5 µM pregabalin alone (21 ± 3%; n = 26) and 2.5 µM gabapentin and 2.5 µM pregabalin applied together (19 ± 2%; n = 9).
In spite of the added complexity of mixed responses with pregabalin, experiments were carried out using fura-2 to measure K + evoked Ca 2+ flux and the effects of both ligands. Our previous experiments with gabapentin (25 µM) had only identified inhibitory effects using this experimental approach [11,12]. Simultaneous application of pregabalin and gabapentin (both at 25 µM) reversibly reduced the K + evoked Ca 2+ influx ( Figure 6B &6C). Pregabalin (25 µM) and gabapentin (25 µM) together reduced the total Ca 2+ flux to 75 ± 5 % (n = 14) of the control response to 30 mM K + . Consistent with the electrophysiological experiments, this level of inhibition was similar to the inhibitory effects of pregabalin and gabapentin applied separately.
The electrophysiological and fura-2 Ca 2+ imaging data show that no additive inhibitory effects were found during simultaneous application of saturating concentrations of gabapentin and pregabalin. The data therefore support the contention that both drugs have a closely related or a common site of inhibitory action. The data also indicate that in the modulation of Ca 2+ channels these drugs do not act in an additive manner even though a substantial proportion of current is resistant to both drugs. It should be emphasized however that pregabalin can reversibly enhance K + -evoked Ca 2+ transients, an effect not seen with gabapentin. Therefore there may be other additional actions of pregabalin that can be identified in Ca 2+ imaging experiments when all membrane conductances are intact.

Does GABA receptor modulation alter responses to pregabalin in cultured dorsal root ganglion neurones?
In this section of the study two strategies were used to evaluate the possible roles of GABA receptors in pregabalin responses in cultured DRG neurones. Firstly, pregabalin actions were studied in the presence of a saturating concentration of GABA; this initially activated all types of GABA receptor and then caused desensitization of these receptors. Secondly, the effect of a potent and selective GABA B receptor antagonist, CGP52432 [19] on pregabalin responses was evaluated. GABA (100 µM) evoked inward currents in a sub-population of cultured DRG neurones and induced a transient rise in intracellular Ca 2+ in 18 of 41 DRG neurones studied (Figure 7A &7B). The inward currents were due to the activation of GABA A receptor Clchannels; with the equilibrium potential for Clunder our recording conditions being close to 0 mV the chloride conductance results in an inward current. Although in vivo the equilibrium potential for Clin DRG neurones is variable, it is predicted to be between -40 mV and -20 mV because of Clloading into the intracellular environment (for review see [20]). Therefore GABA A receptor Clchannel activation can produce a depolarisation of the resting membrane potential. Our data indicated that the GABA-evoked depolarisation was sufficient to result in voltage-activated Ca 2+ channel activity and produce a transient rise in intracellular Ca 2+ . In both neurones that responded to GABA and those that did not, subsequent application of pregabalin, produced either enhancement or inhibition of K + -evoked Ca 2+ influx ( Figure 7A,7B,7C &7D; Table 2). These responses to pregabalin in the presence of GABA were no different in character to the pregabalin responses obtained in the absence of GABA. In neurones that showed a Ca 2+ transient in response to a GABA-evoked depolarisation, there was an apparent increase (to 83%) in the proportion of neurones that were inhibited by pregabalin. It is not clear why this is but it may reflect the sensitivity of Ca 2+ transients evoked in DRG neurones, which can only be evoked consistently in most DRG neurones by three depolarising stimuli. Thus the GABA-evoked responses may influence Ca 2+ homeostatic mechanisms and subsequent stimulated Ca 2+ entry. However, the critical observation from these experiments is that GABA receptor desensitisation does not prevent either Ca 2+ transient enhancement or inhibitory actions of pregabalin in neurones that were sensitive or insensitive to GABA.

Pregabalin and gabapentin do not have additive actions on cultured DRG neurones
The possibility of pregabalin-evoked inhibition of I Ca taking place through activation of G-protein coupled GABA B receptors, was then assessed. CGP52432 (10 µM) when applied alone had no effect on I Ca . In the presence of the GABA B receptor antagonist CGP52432 (10 µM), pregabalin (2.5 µM) evoked a 27 ± 3 % (n = 9) inhibition of the voltage-activated Ca 2+ current and complete recovery was observed 5 minutes after removal of the drug-containing pipette ( Figure 8A). In Ca 2+ imaging experiments 10 µM CGP52432 had no effect on the responses to 25 µM pregabalin. In the presence of CGP52432, 4 out of 16 neurones showed enhanced K + -evoked Ca 2+ influx (to 161 ± 24 % (n = 4) of control) in response to pregabalin and 12 neurones showed inhibition of Ca 2+ influx (64 ± 8 % (n = 12) of control) in response to pregabalin ( Figure 8B &8C).

Is there a role for G-proteins or G-protein coupled receptors in the responses to pregabalin in cultured dorsal root ganglion neurones?
Previously, we found that the actions of gabapentin on I Ca were attenuated by pre-treating DRG neurones with pertussis toxin, an effect that did not appear to involve metabotropic GABA B receptors [12]. In this study several different approaches were taken to examine the influence of receptor and G-protein function in pregabalin actions. Firstly, the effects of a novel thiadiazole compound, SCH-202676, which inhibits ligand binding to a variety of Gprotein coupled receptors (opioid, adrenergic, muscarinic and dopaminergic) were investigated. The selective and reversible action of SCH-202676 appears to involve allosteric modulation of both agonist and antagonist binding to G-protein coupled receptors [21]. Secondly, the potential influences of intracellular flash photolysis of caged GTP-γ-S and subsequent G-protein activation on pregabalin responses were also examined.
SCH-202676 (10 µM) was applied to the intracellular environment via the patch pipette solution. After 5 minutes equilibration the whole cell Ca 2+ current had a mean amplitude of -0.54 ± 0.08 nA (n= 7). During this period there was a clear reduction in the inward current, although a steady state current level was reached. However, subsequent application of pregabalin (2.5 µM) resulted in a further significant reduction in Ca 2+ current amplitude to -0.43 ± 0.07 nA (n = 7, p<0.05). This represents a mean inhibition of 22 ± 5 % by pregabalin, a value very similar to the percentage inhibition produced by pregabalin in the absence of SCH-202676 (21 ± 3%). Therefore SCH-202676 had no effect on the pregabalin responses, indicating that pregabalin was not acting through an SCH-202676-sensitive G-protein coupled receptor (data not shown). Preliminary Ca 2+ imaging experiments were also conducted to determine whether extracellular SCH-202676 (10 µM, continually applied throughout the experiment) altered the effects of pregabalin (2.5 µM) on K + -evoked Ca 2+ transients. Under these experimental conditions pregabalin was found to markedly enhance or inhibit K + -evoked Ca 2+ transients (data not shown). This data again suggests that pregabalin was not acting through mechanisms that were sensitive to SCH-202676.
Caged GTP-γ-S (100 µM) was applied to the intracellular environment via the patch pipette solution and after entering the whole cell recording configuration DRG neurones were left for 5 minutes to equilibrate. Once a stable control Ca 2+ current was obtained, three 200 V flashes of intense near UV light were applied to the neurone to achieve intracellular flash photolysis of the caged GTP-γ-S. We estimate that approximately 15 µM GTP-γ-S was photoreleased by the three flashes. As previously observed intracellular flash photolysis of caged GTP-γ-S reduced the amplitude and slowed the activation of I Ca [22]. The effects of photoreleased GTP-γ-S were attenuated by applying a large depolarising pre-pulse [23], which is consistent with voltage-dependent G-protein modulation of Ca 2+ channels ( Figure 9A). No recovery from photoreleased GTP-γ-S was observed during the period of the experiment. GTP-γ-S and pregabalin (2.5 µM) had additive inhibitory effects on I Ca . These additive actions of GTP-γ-S and pregabalin were apparent regardless of the order of application. One set of experiments was performed in which GTP-γ-S was photoreleased in the intracellular environment and then after stabilisation of the response pregabalin was applied ( Figure 9B). In another set of experiments pregabalin was applied first and after equilibration GTP-γ-S was photoreleased in the continual presence of pregabalin ( Figure 9C). When pregabalin (2.5 µM) was applied first it produced a mean inhibition in I Ca by 25 ± 5 % (n = 4), when applied after photorelease of GTP-γ-S, pregabalin produced a 20 ± 10 % (n = 5) inhibition of I Ca .

Actions of pregabalin on voltage-activated potassium currents
It was clear from the Ca 2+ imaging experiments that not all the cellular actions of pregabalin could be explained by the inhibition of voltage-activated Ca 2+ channels because both inhibition and enhancement in K + evoked Ca 2+ flux was observed. To investigate the actions of pregabalin further, its' effects on voltage-activated K + currents in DRG The GABA B receptor antagonist CGP52432 had no effect on the actions of pregabalin   C neurones were studied. Previously, Stefani and colleagues found that gabapentin modulated neuronal steady state non-inactivating K + currents [18].
Three minutes application of pregabalin (2.5 µM) had no significant effect on the voltage-activated K + current activated from a holding potential of -90 mV by a 100 ms voltage step command to 0 mV ( Figure 10A). However, raising the pregabalin concentration to 250 µM resulted in significant modulation of K + currents in DRG neurones. Pregabalin (250 µM) applied for 3 to 5 minutes produced an enhanced K + current in 11 out of 21 neurones but a modest inhibition of the outward current in 10 out of 21 neurones ( Figure 10B and 10C). DRG neurones are a heterogenous population of neurones and there is evidence that they express at least 6 diverse voltage-activated K + channels and that this expression is in part determined by the type of DRG neurone [24]. To distinguish between the two responses experiments were carried out on DRG neurones held at -30 mV to inactivate a proportion of the outward current. Under these conditions pregabalin inhibited the outward current in 9 out of 11 DRG neurones but still produced enhancement in 2 neurones. The level of inhibition increased at a holding potential of -30 mV to 30 ± 7% (n = 9) compared to 15 ± 4 % (n = 10) at -90 mV, (data not shown).
Interestingly, in apparent contrast to pregabalin, 250 µM gabapentin was initially only found to inhibit K + currents in DRG neurones ( Figure 11A,11B). However, when studies into long-term (10-15 minutes) actions of gabapentin were investigated slowly developing outward current enhancement was identified. So similar to actions of pregabalin, biphasic responses to gabapentin were found with initial inhibition of K + currents and then a delayed enhancement of the outward current ( Figure 11C), [5].
Long-term modulation of K + current by pregabalin was then investigated. Dramatic increases in outward current were observed after a delay. This effect of pregabalin persisted even as pregabalin was removed from the extracellular environment, which may implicate a metabolic or intracellular signalling event in this response. The mean K + current amplitude at +40 mV increased from 3.37 ± 0.73 nA to 7.56 ± 1.10 nA (n = 11; p<0.01) 15 minutes after perfusion of 250 µM pregabalin. Figures 12A and  12B show an individual example record and trace of the delayed response to pregabalin. These responses did reverse but this took about 40 minutes with the response developing 3-10 minutes after the start of pregabalin application. No change in holding current or in the leak conductances were associated with the long-term effect of pregabalin and in the absence of pregabalin stable K + currents were recorded from DRG neurones for 16 minutes (n = 10).
Several pharmacological experimental approaches were taken to characterise the biphasic actions of pregabalin and to determine the possible mechanism of action associated with the long-term K + current modulation. Apamin, a toxin from the honeybee, was used to block small conductance Ca 2+ -activated K + currents. Apamin (1 µM) caused a reduction in outward current at +40 mV from 2.64 ± 0.33 nA to 2.37 ± 0.39 nA, subsequent application of 250 µM pregabalin enhanced the current to 3.02 ± 0.39 nA (n = 11; p<0.01). No inhibitory effects were seen with pregabalin after treatment with apamin, suggesting that it is the apamin-sensitive Ca 2+ -activated K + channels that are inhibited by pregabalin ( Figure 12C &12D).
Gabapentin can be transported into cells via the L αamino acid transporter and may achieve intracellular concentrations 10-20 times the levels in the extracellular environment [25]. Furthermore, actions through intracellular signalling pathways and specifically protein kinase A, have been proposed for gabapentin [12]. To examine whether the long-term enhancement of K + currents by pregabalin might involve intracellular sites of action, we applied pregabalin to the intracellular environments of DRG neurones via the patch pipette solution. Intracellular pregabalin (250 µM) evoked an increase in the K + current that started to develop within the first minute of entering the whole cell recording configuration. After an initial increase that could reflect equilibration with the KClbased patch pipette solution containing pregabalin, a slow sustained significant increase in the outward K + current continued to develop over a 15 minute period (n = 10; p< 0.01; Figure 13A,13B). No similar change in K + current was observed in control studies carried out in the absence of pregabalin ( Figure 13C,13D).
Pertussis toxin pre-treatment results in the uncoupling of sensitive G-proteins from effector mechanisms, including voltage-activated Ca 2+ and K + channels. Additionally, pertussis toxin pre-treatment has previously been found to influence gabapentin actions on I Ca . Pertussis toxin pretreatment (500 ng/ml; 16-18 hours) prevented pregabalin-induced long-term K + current enhancement in cultured DRG neurones (Figure 14A &14B).
The possible role of cAMP-dependent protein kinase A (PKA) in the pregabalin-induced enhancement of K + current was then assessed using the inhibitor (Rp)-cAMP [26]. Intracellular application of 30 µM (Rp)-cAMP applied via the KCl-based patch pipette solution had no effect over a 5 minute equilibration period on voltageactivated K + current (n = 9). This may indicate that PKA has little or no basal or tonic activity on K + currents in DRG neurones in culture. However, when pregabalin was applied to DRG neurones loaded with (Rp)-cAMP no long-term enhancement of the outward current was Pregabalin modulates voltage-activated potassium currents in cultured DRG neurones Acute application of gabapentin produced modest inhibition of voltage-activated K + currents in cultured DRG neurones but long-term measurement of K + currents shows a delayed enhancement in outward current  Control Pregabalin produced delayed enhancement of K + currents in cultured DRG neurones Figure 12 Pregabalin produced delayed enhancement of K + currents in cultured DRG neurones. A) Line graph showing a time course for the delayed action of pregabalin in a single neurone, the open bar shows the period of application of pregabalin (250 µM). Little effect of pregabalin was seen in this neurone until 5 minutes after removal of the pressure ejection pipette containing pregabalin. B) Inset traces show a control K + current activated at +40 mV, modest enhancement of the K + current after 5 minutes application of 250 µM pregabalin and the enhanced outward K + current recorded 9 minutes after application of pregabalin. Apamin mimicked the inhibitory action of pregabalin on K + current but did not prevent enhancement of the K + current by acute application of 250 µM pregabalin. C) Bar chart showing the modest inhibitory effect of apamin (1 µM) on K + current and the enhancement in K + current when pregabalin was applied in the continued presence of apamin (Apamin & PGB). D) Traces showing a control K + current, the inhibited K + current in the presence of 1 µM apamin and the enhanced K + current observed when apamin and pregabalin were applied together. Apamin prevented the inhibitory effect of pregabalin but not the enhancement of K + current. Intracellular application of pregabalin enhanced K + currents in cultured DRG neurones. For these experiments pregabalin was included in the KCl-based patch pipette solution at a concentration of 250 µM. A) Bar chart showing the mean amplitudes of the K + current recorded immediately after entering the whole cell recording configuration (Initial I K ) and the maximum outward current recorded within 16 minutes of entering the whole cell recording configuration (Max I K ). B) Traces of the first K + current recorded using a patch pipette solution containing 250 µM pregabalin (Initial) and from the same cell the maximum outward K + current recorded with intracellular pregabalin (Max). C) Bar chart showing control data recorded from neurones not exposed to pregabalin. Illustrated are the mean amplitudes of the K + current recorded immediately after entering the whole cell recording configuration (Initial I K ) and the maximum K + outward current recorded within 16 minutes of entering the whole cell recording configuration (Max I K ). D) Traces of the first K + current recorded using the standard KCl-based patch pipette solution (Initial) and from the same cell the maximum outward current recorded under control conditions, within 16 minutes of entering the whole cell recording configuration (Max). Pertussis toxin pre-treatment and intracellular (Rp)-cAMP prevented enhancement of K + current by pregabalin. A) Bar chart showing the mean amplitude of K + current recorded from DRG neurones pre-treated with pertussis toxin for 16-18 hours with 500 ng/ml (PTX Control) and after application of 250 µM pregabalin (PTX PGB), long term, up to 15 minutes monitoring of the current. B) Traces showing outward K + currents recorded from a DRG neurone pre-treated with pertussis toxin, prior to pregabalin application (PTX Control) and 10 minutes after application of 250 µM pregabalin. C) Bar chart showing mean data obtained from neurones containing (Rp)-cAMP (30 µM), which was applied to the intracellular environment via the patch pipette solution. Data shows the mean K + current amplitude recorded 1 minute and 5 minutes after entering the whole cell recording configuration (1 min; 5 min), after 5 minutes application of 250 µM pregabalin (PGB) and 10 minutes after removal of the pressure ejection pipette containing pregabalin. D) Traces from a single experiment showing the outward K + currents at 1 and 5 minutes after entering the whole cell recording configuration and allowing entry of 30 µM (Rp)-cAMP in the DRG neurones. Also shown are the K + current inhibited by 5 minutes application of 250 µM pregabalin (PGB) and the recovery of the K + current after the pressure ejection pipette containing pregabalin was removed. Intracellular (Rp)-cAMP prevented the delayed long-term enhancement of the K + current evoked by pregabalin. observed. Under these recording conditions pregabalin still produced some inhibition of the K + current ( Figure  14C &14D). These data provide evidence that the longterm modulation of voltage-activated K + channels by pregabalin is dependent on PKA-mediated phosphorylation.

Conclusions
In conclusion, these results indicate that pregabalin acts via the same basic mechanisms as gabapentin to inhibit voltage-activated Ca 2+ channels and that these inhibitory actions are independent of GABA receptor activation. Some features of the actions of pregabalin on intermediate size and large DRG neurones appear not to be seen with gabapentin. However, these distinct responses involve enhanced K + -evoked Ca 2+ transients by pregabalin rather than inhibition of Ca 2+ channels. Alpha 2 δ subunits of voltage-activated Ca 2+ channels remain a possible site of action for both pregabalin and gabapentin. Dooley and colleagues showed that both gabapentin and pregabalin attenuated K + -evoked norepinephrine release from rat neocortical slices by inhibiting P/Q-type Ca 2+ channels [10]. In cortical pyramidal neurones gabapentin predominantly works through L-type Ca 2+ channels [27]. Our work with Bay K8644 indicates that in cultured DRG neurones gabapentin [5] and pregabalin act predominantly independently of L-type channels. A recent study has also indicated that acting via G-protein coupled GABA B receptors, gabapentin selectively inhibited N-type Ca 2+ channels in hippocampal pyramidal neurones [15]. Interestingly, this selectivity of gabapentin seen in the hippocampus is different from the Ca 2+ channel modulation seen with the GABA B receptor agonist, baclofen. Our previous investigation using "toxityping" showed that gabapentin inhibited a variety of Ca 2+ channels in DRG neurones [11]. We conclude from all this work that gabapentin and pregabalin may have allosteric interactions with promiscuous α 2 δ Ca 2+ channel subunits. These α 2 δ subunits are not specifically combined with distinct pore forming Ca 2+ channel subunits (α 1 ) in all neurones and may therefore inhibit pharmacologically diverse Ca 2+ channels depending on the expression of Ca 2+ channel subunits in different neurones. However, transfection studies have shown that oocytes expressing Ca 2+ channels (Ca v 2.2) containing β1b and α 2 δ-1 or α 2 δ-2 subunits are insensitive to acute application of 50 µM gabapentin [28]. This work raises the possibility that other mechanisms independent of α 2 δ -Ca 2+ channel subunits are involved in the modulation of neuronal excitability by gabapentin. Additionally, the modulation of voltage-activated Ca 2+ channels by gabapentin and pregabalin acting through indirect mechanisms has been suggested. Candidates for such indirect mechanisms include the control of Ca 2+ channel functional expression [29,30] and metabotropic mechanisms linked to pertussis toxin sensitivity and PKA activation [12].
In hippocampal pyramidal neurones, uncoupling G-proteins from metabotropic receptors with N-ethylmaleimide prevents modulation of K + and Ca 2+ channels by gabapentin [15]. However, in DRG neurones it is not clear how pertussis toxin influences the actions of gabapentin and pregabalin but it may involve disruption of multi-protein complexes of G-proteins and ion channel subunits after ADP-ribosylation of Gα. The role of any G-protein coupled receptors in either gabapentin or pregabalin responses in DRG neurones are not supported by our investigations with SCH-202676. Furthermore, receptor and direct G-protein involvement in pregabalin effects on Ca 2+ channels also appears unlikely in DRG neurones. This is because in this study we found that intracellular flash photolysis of GTP-γ-S had no influence on pregabalin-evoked current inhibition and pregabalin did not alter responses to GTP-γ-S. Our findings are in agreement with those made in a previous study on cells expressing GABA B1a/B2 or GABA B1b/B2 receptor subunits. In this study GABA and the GABA B receptor agonist baclofen evoked [ 35 S]-GTP-γ-S binding responses but even at high concentrations both gabapentin and pregabalin did not [31].
The experiments designed to assess potential roles of GABA receptors in the responses to pregabalin showed that GABA receptor desensitization or the blockade of GABA B receptors did not attenuate pregabalin actions. These findings add to the published studies that indicate that gabapentin and pregabalin effects are independent of GABA receptor activation at least in some preparations [31,32]. Specifically, in cultured DRG neurones gabapentin was previously found not to activate a Clconductance to alter membrane potential and input resistance and the GABA B receptor antagonist saclofen did not influence the inhibitory action of gabapentin on I Ca [12].
Pregabalin has a higher affinity for α 2 δ subunits than gabapentin and in a number of studies is more effective. When applied alone pregabalin produced enhancement in Ca 2+ flux in some neurones, an effect not seen with gabapentin. So, it was surprising to see in the imaging experiments that when gabapentin and pregabalin were applied together they produced only inhibitory effects. This may reflect the allosteric interactions between these drugs and α 2 δ subunits of Ca 2+ channels as well as indirect modulation of Ca 2+ dependent conductances and interactions with components of cell signalling.
There appear to be some inconsistencies in the pregabalin data when its actions on voltage-activated Ca 2+ currents are compared with effects on K + -evoked Ca 2+ transients. This may in part be due to effects of pregabalin on mem-brane conductances in addition to voltage-activated Ca 2+ currents. These effects may not be detected under voltage clamp recording conditions where Na + and K + currents are blocked to isolate Ca 2+ currents. In the imaging experiments all voltage-activated conductances are intact and may be modulated by pregabalin. Alternatively, pregabalin could potentially have a direct or indirect influence on Ca 2+ -induced Ca 2+ release from intracellular stores and alter Ca 2+ homeostatic mechanisms. These effects may be modulated differently in different sub-populations of DRG neurones and so produce the mixed responses recorded in small, intermediate and large neurones in this study. Thus different actions of pregabalin may be detected using fura-2 fluorescence imaging but these additional mechanisms may not influence the measurements of Ca 2+ currents. Although mixed responses to gabapentin were not previously identified, neither were clear effects on the amplitude of K + -evoked Ca 2+ transients apparent in DRG neurones [11], although they were seen in differentiated F-11 cells [12]. These apparent anomalies seen with pregabalin do not appear to be due to drug effects on Ca 2+ -induced Ca 2+ release or Ca 2+ homeostatic mechanisms such as Na + /Ca 2+ exchange. This conclusion was reached because pregabalin did not influence Ca 2+ transients evoked by caffeine and still enhanced K + -evoked Ca 2+ transients in choline chloride-based (low Na + ) extracellular solution.
The mixed responses to pregabalin seen in the Ca 2+ imaging experiments prompted us to investigate the actions of pregabalin on voltage-activated K + currents, which could be modulated to cause an increase in Ca 2+ influx. This speculation was supported by the finding that application of the K + channel inhibitor, TEA, enhanced K + -evoked Ca 2+ transients. Stefani and colleagues have reported inhibition of outward K + currents by gabapentin [18]. Additionally, in rat hippocampal and human neocortical brain slices gabapentin has been shown to inhibit K + -evoked [ 3 H]-noradrenaline release. The activation of K ATP channels is implicated in these responses because not only does glibenclamide, a K ATP channel antagonist, attenuate the gabapentin response but also pinacidil, a K ATP channel agonist, mimics the response and did not have additive effects with gabapentin [33].
Mixed or biphasic responses to gabapentin and pregabalin were seen, with both inhibition and enhancement of K + currents observed. The inhibitory actions of both gabapentin and pregabalin on K + currents recorded from DRG neurones may not reflect a direct action of these drugs on K + channels. The effects of apamin, which prevented the inhibition of outward current by pregabalin indicated that small conductance Ca 2+ -activated K + channels were involved in the response. This is consistent with the effects of gabapentin seen in isolated cortical neurones [18]. The pregabalin inhibitory action on voltage-activated Ca 2+ channels may underlie the inhibition of outward K + current. A reduction in Ca 2+ influx would lead to reduced activation of Ca 2+ -activated K + channels and therefore a smaller outward current. If this indirect effect of pregabalin resulted in a disproportionate reduction in Ca 2+ -activated K + conductances this may result in prolonged depolarisation. In turn a prolonged depolarisation may maintain activation of Ca 2+ channels that are insensitive to pregabalin and an increase in Ca 2+ influx as seen in a sub-population of larger DRG neurones in the Ca 2+ imaging experiments. It is not clear why gabapentin, which produced modest inhibition of K + currents, did not also produce enhancement of K + -evoked Ca 2+ transients. It may be explained by a balance between inhibition of Ca 2+ influx through voltage-activated channels and modulation of Ca 2+ flux through inhibition of K + conductance. Consistent with this hypothesis, gabapentin appears to be less effective than pregabalin at modulating K + -evoked Ca 2+ transients in DRG neurones. This is supported by our previous study that showed that gabapentin did not significantly alter the peak Ca 2+ transients [11].
The delayed enhancement of voltage-activated K + currents by gabapentin and pregabalin is a powerful mechanism for reducing cell excitability. The slow development of this response and its gradual decline suggested that intracellular signalling events were involved. Pregabalin produces enhancement of K + currents when applied either inside or outside DRG neurones. The delay in the development of these responses is less with intracellular application of pregabalin, suggesting that it may have an intracellular site of action. This effect may also depend on drug uptake into cells via L α-amino acid transporters and the presence and activity of these transporters may greatly influence cell sensitivity to gabapentin and pregabalin. Although it is not clear how gabapentin and pregabalin might activate PKA, it is a candidate target site. Our previous work showed that inhibition of Ca 2+ currents by gabapentin was sensitive to cAMP analogues that activated or inhibited PKA [12]. Similarly, in this present study (Rp)-cAMP blocked enhancement of K + currents by pregabalin. In the literature there are a number of reports of PKA altering neuronal excitability and modulating K + conductances. PKA is involved in pain signalling, playing a role in prostaglandin-induced activation and sensitization of DRG neurones [34]. PKA activity attenuates KV3.2 channel currents [35] and A-type current [36] and is involved in the inhibition of K + conductances by prostaglandin E-2 [37]. Our results are not explained by these findings but PKA has also been implicated in presynaptic inhibition of GABA release [38], large conductance calcium -and voltage-activated K + channel activity [39] and cannabinoid receptor mediated modulation of ion channels [40]. In the context of our project, of particular inter-est is the biphasic modulation in retinal cones of voltage dependent K + and Ca 2+ channels by a synthetic cannabinoid receptor agonist and the sensitivity of these responses to Wiptide, a PKA inhibitor [40]. However, the G-protein pharmacology is different in the cone cells suggesting different PKA activation pathways in DRG neurones exposed to pregabalin. There appears to be a considerable number of apparently conflicting effects mediated by PKA, although a number of different preparations have been studied. The concept of conditional protein phosphorylation such that initial phosphorylation state affects the sensitivity of different effector targets to subsequent activation [39] could determine responses to gabapentin and pregabalin acting through PKA. A future challenge will be to determine how PKA plays roles in activation and sensitization of DRG neurones and also plays a role in gabapentin and pregabalin responses to dampen down electrical excitability in the same neurones. Perhaps answers will come when we have a better understanding of the changes in neuronal phenotype that develop with pain disorders?
An interesting feature of some actions of gabapentin and pregabalin is that their effects often outlast the period of drug delivery. Maintained intracellular signalling effects of these drugs and the delayed responses involving PKA modulation of Ca 2+ and K + conductances may underlie this characteristic.
Gabapentin has previously been shown to attenuate high frequency action potential firing both in CNS neurones [41] and in cultured DRG neurones [5,12]. Pregabalin produces the same effect, dramatically but reversibly reducing the number of action potentials fired in response to 300 ms depolarising current commands. The underlying mechanisms involved in these reductions in electrical excitability could involve inhibition of voltage-activated Ca 2+ channels with resulting reduced activation of Ca 2+activated K+ currents and effects on voltage-dependent ion channel availability. Although the delayed enhancement of voltage-activated K + channels could contribute, this effect appears to develop over a longer period. These actions may contribute both to anticonvulsant effects of gabapentin and pregabalin as well as their therapeutic actions in pain disorders. It is particularly worth noting that the inhibition of Ca 2+ influx is most consistently seen in small diameter cultured DRG neurones that are likely to be the pain fibres.
Our data adds to previous work that has assessed the actions of gabapentin and pregabalin on K + -evoked neurotransmitter release in CNS neurones [10] and the pharmacology of pain in whole animal models [8,9,[42][43][44]. The present work and these previous studies suggest that gabapentin and pregabalin mostly act through the same basic mechanisms. These mechanisms in cultured DRG neurones appear to involve allosteric inhibitory modulation of Ca 2+ channels through drug interactions with α 2 δ subunits, reduced activation of Ca 2+ -dependent conductances and modulation of both Ca 2+ and K + channels through metabotropic mechanisms that involve PKA. Interestingly, both gabapentin and pregabalin inhibit release of sensory peptides (substance P and CGRP) this may provide a mechanism of action down-stream of ion channel modulation. This effect is only observed after inflammation and may contribute to their analgesic properties of gabapentin and pregabalin [45].
Ca 2+ channels in DRG neurones are targets for antinociceptive agents but the pharmacology of these channels remains to be fully exploited in pain therapy [46,47]. Further studies on changes in ion channel subunit expression and alterations in intracellular signalling pathways in pain disorders may in the future open up new therapeutic opportunities.

Cell culture
One to four-day old Sprague-Dawley rats were decapitated and dorsal root ganglia removed. DRG neurones were dissociated enzymatically (0.125% collagenase for 13 minutes and 0.25% trypsin for 6 minutes) and mechanically (trituration). Primary cultures of DRG neurones were plated on lamin-polyornithine coated coverslips and bathed in Ham's F-14 culture medium (Imperial Laboratories) containing 10% horse serum (Gibco), low NGF (20 ng/ml; Sigma), NaHCO 3 (14 mM), streptomycin (50 µg/ml) and penicillin (50 IU/ml). The cultures were maintained for up to two weeks at 37°C in humidified air with 5% CO 2 . Cultures were re-fed with fresh media after 5 days.
In some experiments DRG neurones were pre-treated with pertussis toxin (500 ng/ml; for 18 hours) to ADP-ribosylate the α subunits of certain G-proteins. This prevents pertussis toxin-sensitive G-protein being activated through a range of G-protein coupled receptors and inhibits coupling to voltage-activated Ca 2+ channels and other potential effectors [12,48].

Electrophysiology and calcium imaging
The whole cell patch clamp recording method and fura-2 Ca 2+ imaging were used to measure the inhibitory actions of pregabalin and gabapentin on Ca 2+ entry through voltage-activated channels. Initially, multiple firing properties of a sub-population of DRG neurones were studied using a patch pipette solution containing in mM: KCl, 140; EGTA, 5; CaCl 2 , 0.1; MgCl2, 2.0; HEPES, 10.0; ATP, 2.0. The extracellular solution containing in mM: NaCl, 130; KCl, 3.0; CaCl 2 2.0; MgCl 2 , 0.6; NaHCO 3 1.0, HEPES 10.0 and glucose 5.0. For recording Ca 2+ channel currents the patch pipettes were filled with CsCl-based solution containing in mM: 140 CsCl, 0.1 CaCl2, 5 EGTA, 2 MgCl2, 2 ATP, 10 Hepes. The pH and osmolarity of the patch pipette solutions were corrected to 7.2 and 310-320 mOsm.l -1 with Tris and sucrose. The extracellular bathing solution used to study Ca 2+ currents contained in mM: 130 choline chloride, 2 CaCl2, 3 KCl, 0.6 MgCl2, 1 NaHCO3, 10 HEPES, 5 glucose, 25 tetrethylammonium chloride, 0.0025 tetrodotoxin (Sigma). The pH and osmolarity of this extracellular bathing solution was corrected to 7.4 and 320 mOsml -1 with NaOH and sucrose respectively. The recording solutions used in these experiments were designed to attenuate voltage-activated Na + and K + currents and isolate voltage-activated Ca 2+ currents. The range of values for the series resistance under our recording conditions was from ~8 to 15 MΩ. Pregabalin and other drugs were applied to the extracellular environment by low-pressure ejection from a blunt pipette positioned about 50-100 µm away from the cell being recorded. Voltage-activated Ca 2+ currents were evoked by 100 ms voltage step commands applied every 30 s. Similar protocols were used to study the actions of pregabalin on voltage-activated K + currents but NaCl-based extracellular bathing solution and KCl-based patch pipette solutions were used.
In some experiments GTP-γ-S was photoreleased inside DRG neurones. Caged GTP-γ-S (100 µM; Molecular Probes) was included in the CsCl-based patch pipette solution. After obtaining control Ca 2+ currents intracellular flash photolysis was achieved by flashing the neurone being studied (three 200 V flashes to producẽ 15µM GTP-γ-S) using a XF-10 xenon flash lamp with a UG11 bandpass filter (Hi-Tech Scientific; [49]) All voltage-activated Ca 2+ and K + currents had scaled linear leakage and capacitance currents subtracted to obtain values for the net inward Ca 2+ current or net outward K + current. Data are given as mean ± standard error of the mean (s.e.m.) values and statistical significance was determined using a paired or independent Student's t test as appropriate.
For Ca 2+ imaging in cultured DRG neurones the cultures were incubated for 1 hour in NaCl-based extracellular solution contained (in mM): NaCl, 130; KCl, 3.0; MgCl 2 , 0.6; CaCl 2 , 2.0; NaHCO 3 , 1.0; HEPES, 10.0; glucose, 5.0 and fura-2AM, 0.01; (Sigma, 1 mM stock in dimethylformamide). The pH was adjusted with NaOH to 7.4 and the osmolarity to 310-320 mOsm with sucrose. The cells were then washed for 10-20 minutes with NaCl-based extracellular solution to remove the extracellular fura-2AM and this period allowed cytoplasmic de-esterifica-tion of the Ca 2+ sensitive fluorescent dye. The cells were constantly perfused with NaCl-based extracellular solution (1-2 ml/min) and viewed under an inverted Olympus BX50WI microscope with a KAI-1001 S/N 5B7890-4201 Olympus camera attached. Some Ca 2+ imaging experiments were carried out using a Ca 2+ -free NaCl-based solution (as standard NaCl-based extracellular solution but with no added CaCl 2 ) or choline chloridebased solution (as standard NaCl-based extracellular solution but with choline chloride in place of NaCl). The fluorescence ratiometric images from data obtained at excitation wavelengths of 340 nm and 380 nm were viewed and analysed using OraCal pro, Merlin morphometry temporal mode (Life Sciences resources, version 1.20). The DRG neurones were stimulated with NaClbased extracellular solution containing high K + (30 mM), which produced depolarisation, activation of voltagegated Ca 2+ channels and large transient increases in intracellular Ca 2+ . Three consistent transient increases in intracellular Ca 2+ could be obtained in a single experiment on cultured DRG neurones [11]. The actions of pregabalin and gabapentin (2.5-250 µM) were investigated on the response to the second stimulus in DRG neurones. The actions of pregabalin and gabapentin on the Ca 2+ transient amplitude, duration at 1/2 peak amplitude and total Ca 2+ flux were measured. All experiments were conducted at room temperature and data are expressed as means ± s.e.m.