Figure 5
From: A covalent peptide inhibitor of RGS4 identified in a focused one-bead, one compound library screen
Dose curves of 5nd on various RGS4 cysteine mutants. Various concentrations of 5nd were tested for disruption of the RGS4-Gαo interaction for C95A (5 nM), or C148A (5nM) (A), C71A (5nM) or C148A (5nM) (B), A95C in the -7C (5nM), or A132C in the -7C (5nM) (C), A71C in the -7C (5nM), or A148C in the -7C (5nM) (D) or the mutant with no cysteines in the domain (20 nM) or the C-terminus (20 nM) (E) in the FCPIA (mean ± S.E.M., n ≥ 3). See Table 2 for statistics.