Figure 4
GSH, CYS, GSSG levels, and GSH/GSSG ratio in H9c2 cells after treatment with DOX in presence or absence of NACA. H9c2 cells were plated in 75 cm2 tissue culture flasks at a density of 4 × 104 cells/cm2. Cells were treated with or without NACA for 2 h. Then the cell medium was discarded and freshly prepared cell medium containing 5 μM DOX in presence or absence of 750 μM NACA was used to treat the cells for a period of 24 h. Values are mean ± SD from three independent experiments performed in quadruplicate. Significance indicated by: *p < 0.05 compared to control group; ** p < 0.05 compared to the DOX-only group.