Table 3 Binding parameters of NMS, ACh and rapacuronium to membranes from CHO cells expressing M1 through M5 receptor subtypes.
From: Divergence of allosteric effects of rapacuronium on binding and function of muscarinic receptors
[3H]NMS | [3H]Ach | Ach | ||||
|---|---|---|---|---|---|---|
high affinity binding | low affinity binding | |||||
pKA | pα | pKA | pα | pKA | pβ | |
M1 | 5.37 ± 0.03 | -1.08 ± 0.05 | 5.37 ± 0.04 | -1.30 ± 0.07 | 5.33 ± 0.03 | -1.32 ± 0.06 |
M2 | 5.59 ± 0.03 | -0.83 ± 0.07 | 5.55 ± 0.05 | -1.46 ± 0.08 | 5.56 ± 0.04 | -1.52 ± 0.06 |
M3 | 4.75 ± 0.04 | -1.11 ± 0.05 | 4.80 ± 0.04 | -1.26 ± 0.05 | 4.77 ± 0.05 | -1.20 ± 0.07 |
M4 | 5.42 ± 0.04 | -1.40 ± 0.04 | 5.41 ± 0.05 | -1.51 ± 0.05 | 5.49 ± 0.04 | -1.56 ± 0.07 |
M5 | 4.97 ± 0.04 | -1.54 ± 0.05 | 4.92 ± 0.03 | -1.34 ± 0.07 | 4.95 ± 0.04 | -1.28 ± 0.07 |