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Table 2 Effects of rapacuronium on the rate of [3H]NMS dissociation.

From: Divergence of allosteric effects of rapacuronium on binding and function of muscarinic receptors

 

Control

+ 100 μM rapacuronium

 

koff [min-1]

koff1 [min-1]

f2 [%]

koff2 [min-1]

M1

0.063 ± 0.004

0.014 ± 0.001*

14 ± 3

0.34 ± 0.05

M2

0.18 ± 0.01

0.026 ± 0.002*

23 ± 5

0.76 ± 0.11

M3

0.048 ± 0.003

0.031 ± 0.002*

20 ± 4

0.058 ± 0.009

M4

0.041 ± 0.002

0.017 ± 0.001*

6.0 ± 2.0

0.75 ± 0.11

M5

0.013 ± 0.001

0.0078 ± 0.0004*

  
  1. Observed rates of [3H]NMS dissociation (koff, koff1 and koff2) and fraction of sites (f2) with faster dissociation (koff2) from individual subtypes of muscarinic receptors were obtained by fitting Eq. 7a and 7b to data in Figure 3. Results of better fit are shown. Values are means ± SE of fits to 3 independent experiments performed in quadruplicates. *P < 0.05; significantly different from control ([3H]NMS alone) by t-test
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BMC Pharmacology

ISSN: 1471-2210