Figure 9

Effect of rapacuronium on the time course of [³H]ACh dissociation. Membranes from CHO cells expressing individual subtypes of muscarinic receptors were prelabeled with 40 nM [³H]ACh for 60 min. At time zero 40 μM unlabeled ACh was added alone (closed circles) or as a mixture with 1 μM rapacuronium (open circles) or 10 μM rapacuronium (hatched circles). Incubations were terminated at the times indicated on the x-axis. Specific [³H]ACh binding is expressed as percent of specific binding at time 0 on x-axis. Data are means ± SE of values from 3 independent experiments performed in quadruplicates. Binding parameters are shown in Table 6.