Figure 7
From: Divergence of allosteric effects of rapacuronium on binding and function of muscarinic receptors
Effects of rapacuronium on kinetics of [35S]GTPγS binding. Membranes were preincubated for 60 min in the presence (open symbols) or absence (closed symbols) of 1 μM rapacuronium. Then [35S]GTPγS was added simultaneously with buffer (circles) or 10 μM ACh (squares). Incubations were terminated at the times indicated on the x-axis. The increase of specific [35S]GTPγS binding is expressed as fmol per μg of protein (left) and as fold increase of specific binding under basal conditions (right). Data are means ± SE of values from 3 independent experiments performed in quadruplicates.