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Figure 6 | BMC Pharmacology

Figure 6

From: Divergence of allosteric effects of rapacuronium on binding and function of muscarinic receptors

Figure 6

Effects of rapacuronium on ACh-induced [35S]GTPγS binding to membranes. Membranes from CHO cells expressing individual subtypes of muscarinic receptors or nontransfected CHO-K1 cells were preincubated for 60 min with buffer (closed symbols) or with rapacuronium (open symbols). [35S]GTPγS binding was induced by rapacuronium alone (closed squares), ACh alone (closed circles), or by ACh in the presence of rapacuronium (0.1 μM (open circles), 1 μM (open squares), or 10 μM (open triangles)). Data are expressed as fold increase of basal [35S]GTPγS binding and are presented as means ± SE from 3 independent experiments performed in quadruplicates. Curves are fits of Eq. 5 to data. Parameters are summarized in Tables 4 and 5.

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