Figure 4

Effects of rapacuronium on high-affinity [³H]ACh binding. Binding of 20 nM [³H]ACh to membranes from CHO cells expressing individual subtypes of muscarinic receptors (circles, M₁; squares, M₂; diamonds, M₃; up-triangles, M₄; down-triangles, M₅) was determined in the presence of rapacuronium at the concentrations indicated on the x-axis and is expressed as percent of specific binding in the absence of rapacuronium. Data are means ± SE from 3 independent experiments performed in quadruplicates. Curves are fits of Eq. 3 to data. Binding parameters are summarized in Table 3.