Figure 3

Effects of 100 μM rapacuronium on dissociation of [³H]NMS binding. Binding of [³H]NMS to membranes from CHO cells expressing individual subtypes of muscarinic receptors at different times after the addition of 10 μM NMS (closed circles) or a mixture of 10 μM NMS and 100 μM rapacuronium (open circles). Specific binding is expressed as percent of binding at time 0. Data are means ± SE from 3 independent experiments performed in quadruplicates. Binding parameters are summarized in Table 2.