Figure 1

Saturation binding of [³H]NMS and [³H]Ach. Specific binding of [³H]NMS (circles) and [³H]ACh (squares) to membranes from CHO cells expressing individual subtypes of muscarinic receptors is plotted against the concentration of free radioligand. Binding of radioligand in the absence (closed symbols) and presence of 10 μM (open symbols) or 100 μM (hatched symbols) rapacuronium, respectively. Data are means ± SE from 3 independent experiments performed in quadruplicates. Curves are fits of Eq. 1 to data. Binding parameters are summarized in Table 1.