Figure 7
From: Differential regulation of muscarinic M1 receptors by orthosteric and allosteric ligands
Continued activation of M 1 receptors after agonist treatment. CHO-hM1 cells were treated with agonists for 1 minute prior to washing and incubation in the presence of 10 mM LiCl2. Total IP accumulation after 1 hour was measured as described under Methods. Data presented are mean ± SD for duplicate measurements from a representative experiment repeated twice with similar results. Statistically significant difference compared to no drug, * = p < 0.05, (Student t-test, GraphPad, Prism).