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Figure 5 | BMC Pharmacology

Figure 5

From: Differential regulation of muscarinic M1 receptors by orthosteric and allosteric ligands

Figure 5

Down-regulation of Muscarinic M 1 Receptors in CHO Cells. (A) Total receptor binding remaining following 24 hours of treatment with various agonists, in the presence or absence of the muscarinic antagonist atropine, was measured in CHO cells stably expressing hM1 receptors. The data presented are mean ± S.D from 3-6 pooled experiments performed in triplicate. Total binding is expressed as % of control at 24 hours. Total receptor binding remaining after treatment with each ligand was; No drug, 100% ± 3, carbachol, 49% ± 3, xanomeline, 48% ± 12, NDMC, 49% ± 6, AC-42, 56% ± 7, AC-260584, 56% ± 4. Statistically significant difference compared to no drug, * = p < 0.001, (Student t-test, GraphPad, Prism). (B) No loss in [3H]-QNB binding could be measured when the cells were treated with the same concentration of each ligand for only 2 hours, confirming proper removal of ligands during washing.

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