Figure 5From: Spiperone enhances intracellular calcium level and inhibits the Wnt signaling pathwayThe protein kinase C inhibitor GF109203X does not affect spiperone-mediated inhibition of Wnt signaling. A TOPflash reporter was transfected into HEK293 cells with expression plasmids for Wnt1, LRP6 and Dvl as indicated in figure. After transfection, cells were treated with 5 μM spiperone, 5 μM GF109203X and combined use of two drugs for another 24 h. Then luciferase values were determined.Back to article page